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Lab-on-a-Chip-Based Devices for Rapid and Accurate Measurement of Nanomaterial Toxicity
Published in Suresh C. Pillai, Yvonne Lang, Toxicity of Nanomaterials, 2019
Mehenur Sarwar, Amirali Nilchian, Chen-zhong Li
The fabrication process started with using a double-sided, polished, 4-inch quartz glass wafer (University Wafers, USA). This wafer was then immersed in piranha solution for 30 minutes. After that, the wafer was rinsed thoroughly with de-ionised water and dried with nitrogen gas. To remove any residual liquid from the wafer, it was then placed on a hot plate for 5 minutes at 115°C. An ion beam evaporator (JEOL, Japan) was used to deposit 25 nm of Cr adhesion layer and 250 nm of Au thin films. AZ1518 (MicroChem Co.) positive photoresist is spin-coated on the wafer and exposed to a pattern glass-chrome mask using a mask aligner (OAI 800). The developer, AZ400 used with DI water in a ratio of 1:4. Etching was performed by an Au etchant and Cr etchant to remove undesired Au and Cr, respectively. It was further cleaned under sonication for 5 minutes each time in solvents like Acetone, Methanol, and DI water. Finally, a plasma cleaning step was done in a reactive oxygen chamber.
In vivo impact assessment of orally administered polystyrene nanoplastics: biodistribution, toxicity, and inflammatory response in mice
Published in Nanotoxicology, 2021
Yun Ju Choi, Jun Woo Park, Yong Lim, Sungbaek Seo, Dae Youn Hwang
The whole liver, kidney, and intestine tissue samples of mice were homogenized with Polytron® Homogenizer PT 3100 D (KINEMATICA, Luzern, Switzerland), and centrifuged at 10,000 rpm (1,411 × g) for 5 min (C1015 micro prime centrifuge; Centurion Scientific Ltd, Stoughton, Chichester, UK). The resultant supernatant was discarded, and the remaining pellet was digested in 2 mL of piranha solution (H2SO4:H2O2 = 7:3) for 4 h at room temperature. After centrifugation, the supernatant was discarded and the pelleted beads were resuspended in 2 mL DI water. The fluorescence of PS NP was measured using a fluorescence reader (Spectramax i5D, Molecular Devices, San Jose, CA). The intensity of PS NP in each tissue was calculated as follows:
Effect of silica nano-spheres on adhesion of oral bacteria and human fibroblasts
Published in Biomaterial Investigations in Dentistry, 2020
Pawel Kallas, Hua Kang, Håkon Valen, Håvard Jostein Haugen, Martin Andersson, Mats Hulander
Standard microscope glass slides were treated in an UV-O3 cleaner (BHK INC., Claremont, CA) for 15 min to remove organic contaminants and then washed in basic piranha solution (MQ water, NH4OH and 30% H2O2; 5:1:1) for 15 min at 80 °C. After that, surfaces where rinsed with MQ water and dried under N2 (g) flow. Immediately after, the surfaces were placed in a sealed container together with 3-(ethoxydimethylsilyl)propylamine in methanol (50/50; 200 µl each) in a watch glass for 30 min to amine-functionalize the substrates through evaporation of the silane onto the surfaces. Surfaces with homogenous distribution of nanoparticles were then prepared by submerging only half of the amine functionalized surface into a colloidal solution with 40 nm sized SiO2 nanoparticles (∼10 nM nanoparticle concentration) in 5 mM sodium citrate buffer (pH 4) for 15 min before thoroughly rinsing with MQ water and drying under a stream of N2 (g).
Method article: an in vitro blood flow model to advance the study of platelet adhesion utilizing a damaged endothelium
Published in Platelets, 2022
Alison Leigh Banka, Omolola Eniola-Adefeso
Glass coverslips were cleaned with Piranha solution (3:1 concentrated sulfuric acid: 30% hydrogen peroxide) and silanated with 2% 3-(trimethoxysilyl)propyl methacrylate (MPS) by volume in 95% ethanol for 30 minutes. The cleaned coverslips were coated with 1 mg/mL collagen or gelatin for 2 hours at room temperature; the entire coverslip surface was coated with collagen or gelatin. After the 2-hour incubation, the coverslips were rinsed with PBS, then attached to the PFFC and perfused with blood as described above.