Cauterization

Cauterization

Visualizing Hepatic Immunity through the Eyes of Intravital Microscopy

Published in Margarida M. Barroso, Xavier Intes, In Vivo, 2020

Maria Alice Freitas-Lopes, Maísa Mota Antunes, Raquel Carvalho-Gontijo, Érika de Carvalho, Gustavo Batista Menezes

Throughout the procedure, it is essential that the animal remains warm, hydrated, and sedated. After complete sedation, attach the mouse on the surgical stage using a piece of adhesive tape (Figure 16.3A). After fixation, the animal will undergo laparotomy to expose the liver. To do this, apply mineral oil to the abdominal skin to avoid imaging interference by small hairs in the imaging field. Alternatively, the mouse may be shaved before the experiment. The next step is laparotomy. Using surgical scissors and forceps, perform the midline incision in the abdomen from the pubis to the xiphoid process (Figure 16.3B). Dissociate the abdominal musculature from the skin and cauterize the vessels in the field of view (Figure 16.3C, D). The correct and complete cauterization of the vessels is a critical step because it will prevent bleeding.

Sonication to improve the yield in culture-negative peri-prosthetic joint infection

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Journal Information

Published in Egyptian Journal of Basic and Applied Sciences, 2019

Noha Tharwat Abou El-Khier, Samah Sabry El-Kazzaz, Adham Elgeidi, Abd El Rhman Elganainy

Despite the existence of definite signs of infection, cultures may be negative in 7–39% of PJI cases. This occurs due to many factors; patchy distribution of infection, low inoculum of infection, the lack of sensitivity and specificity of standard culture methods because of sampling limitations (i.e. excessive cauterization), the presence of non-recoverable biofilm-embedded bacteria on the prosthetic material, fastidious organisms, and the prior antibiotic therapies [9,10].

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Visualizing Hepatic Immunity through the Eyes of Intravital Microscopy

Sonication to improve the yield in culture-negative peri-prosthetic joint infection