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Corrosion Behaviour of a Carbon Steel Valve in a Microbial Environment
Published in A. K. Tiller, C. A. C. Sequeira, Microbial Corrosion, 2021
J. C. Danko, C. D. Lundin, N. J. E. Dowling, W. Hester
This is a standard procedure described by the American Society for Microbiology manual of methods for general bacteriology (Gerhardt, 1981). The data give a reasonably accurate measurement of the total numbers of bacteria involved and some distinction as to morphology, for example, whether a distinctive organism such as Gallionella is present. 1 mL of each sample was added to 9 mL of sterile mineral salts and vortexed. 1 mL of this dilution was then delivered to a Nucleopore™ 0.2 μm pore-size filter. The filter was pre-stained with Irgalan black to reduce autofluorescence. A solution of 0.01% Acridine Orange was then applied to the filter for 2 min and washed through with sterile tap water. The filters were then recovered from the filtration manifold, dried, and supported on a glass slide with a drop of optically pure immersion oil. Subsequent microscopy was with a ZEIS epifluorescent microscope.
The Human Microbiome: How Our Health is Impacted by Microorganisms
Published in Michael Hehenberger, Zhi Xia, Huanming Yang, Our Animal Connection, 2020
Michael Hehenberger, Zhi Xia, Huanming Yang
Although our understanding of the relationship between intestinal microbes and human health has evolved, many problems still need to be solved. For example, a large number of gut microbes cannot yet be cultivated alone. However, it is believed that in the near future, the gut microbiological code that affects the health of microbes will be cracked one by one. Several international research collaborations have been established, among them the European Metagenomics of the Human Intestinal Tract (MetaHIT101) project, the NIH-funded Human Microbiome Project102 (HMP), and the Earth Microbiome Project103 (EMP). Realizing a healthy body by regulating intestinal microbes will no longer be a dream, as interdisciplinary research into zoology, microbiology, bacteriology, genetics, and medicine will generate breakthroughs that promise to have a revolutionary impact on human health.
The Human Microbiome: How Our Health is Impacted by Microorganisms
Published in Michael Hehenberger, Zhi Xia, Our Animal Connection, 2019
Although our understanding of the relationship between intestinal microbes and human health has evolved, many problems still need to be solved. For example, a large number of gut microbes cannot yet be cultivated alone. However, it is believed that in the near future, the gut microbiological code that affects the health of microbes will be cracked one by one. Several international research collaborations have been established, among them the European Metagenomics of the Human Intestinal Tract (MetaHIT101) project, the NIH-funded Human Microbiome Project102 (HMP), and the Earth Microbiome Project103 (EMP). Realizing a healthy body by regulating intestinal microbes will no longer be a dream, as interdisciplinary research into zoology, microbiology, bacteriology, genetics, and medicine will generate breakthroughs that promise to have a revolutionary impact on human health.
Spring water quality monitoring using multiple bioindicators from multiple collection sites
Published in Journal of Toxicology and Environmental Health, Part A, 2023
Igor Romeiro dos Santos, Isabela Náthaly Machado da Silva, Carlos Filipe Camilo-Cotrim, Luciane Madureira de Almeida, Leonardo Luiz Borges, Elisa Flávia Luiz Cardoso Bailão
Industrial-process products are released into river systems without adequate treatment (Caixeta, Bravo, and Pereira 2022; Carmalin and Lima 2018). However, the impact of emerging pollutants on springs is poorly understood from an ecotoxicological point of view. Water quality parameters are important tools to measure the degree of pollution of water bodies. One water quality parameter is quantifying bacterial populations. Heterotrophic bacteria quantification has been used in sanitary bacteriology to determine drinking water quality. Heterotrophic bacteria in large numbers increase the likelihood of pathogenic bacteria that result in infections (Reddy et al. 2022). The rise of heterotrophic bacteria in the Extrema River spring, greater than 500 colonies/mL at the three sampled sites, indicates that the spring water is unfit for consumption and requires adequate treatment. However, individuals were found using the Extrema River water for recreational purposes, such as swimming, which might lead to this ingestion of this water.
Lawsonia inermis-loaded poly (L-lactide-co-D, L-lactide) nanofibers for healing acceleration of burn wounds
Published in Journal of Biomaterials Science, Polymer Edition, 2023
Susan Bayati, Parmida Harirchi, Payam Zahedi, Abdolmajid Bayandori Moghaddam
Poly (L-Lactide-co-D, L-Lactide) (grade Resomer LR708; 70:30, average molecular weight 1.5 × 106 Da with highly degradable) was purchased from Evonik Germany. The henna-seeded leaves were collected from the village of Chahestan, Bandar Abbas, Iran. Chloroform solvent (molecular weight 119.38 g/mol and density 1.48 kg/liter, chemical solvent dimethylformamide (molecular weight 73.10 g/mol and density of 0.948 kg/liter), potassium dihydrogen phosphate with weight Molecular weight of 136.08 g/mol and potassium hydroxide with a molecular weight of 56.11 g/mol were obtained from Merck, Germany. Bacterial strains with the names Staphicolus aureus (S. aureus) (ATCC 25923) and Pseudomonas aeruginosa (P. aeruginosa) (ATCC 27853) maintained at the Bacteriology Department Faculty of Microbiology, University of Tehran, was used. Comfeel Plus wound dressing product by Coloplast Co., Denmark, was used for comparison. All solvents used were purchased in neat form.
Optimization and Simulation of Process Parameters in Biosorption of Heavy Metals by Alcaligenes faecalis Strain UBI (MT107249) Isolated from Soil of Local Mining Area in North-West Nigeria
Published in Soil and Sediment Contamination: An International Journal, 2022
Umar Balarabe Ibrahim, Sani Yahaya, Ibrahim Yusuf, Abdullahi Hassan Kawo
The identity of the isolate was detected by studying its morphological, biochemical and molecular characteristics. The Gram-staining was employed to determine the cell wall morphology. Biochemical tests carried out during the identification processes include catalase test, oxidase test, nitrate reduction test, urease production, methyl-red reaction, Voges-Proskauer test, indole and motility test. Bergey’s Manual of Determinative Bacteriology was used as an aid to classify the bacteria identified (Brenner et al. 2005). The 16SrRNA gene sequence was obtained by PCR using the universal bacterial primers 27 F (5ʹ-AGAGTTTGATCMTGGCTCAG-3ʹ) and 685 R (5ʹ-TCTACGCATTTCACCGCTAC-3ʹ). The final PCR mixture (50 µl) consisted of 0.4 μM of each primer, 250 µM of each of dNTP, 1 U Dynazyme Taq DNA polymerase (Finnzymes Reagents, Thermo Fisher Scientific, Inc. Finland), 1 x PCR Dynazyme buffer with final MgCl2 concentration of 1.5 mM, and 1 µl of the template DNA solution. The temperature program consisted of an initial denaturation at 94°C for 1 minutes, 30 cycles (94°C for 30 seconds, 60°C for 30 seconds, 72°C for 1 minute) and a final extension step was carried out at 72°C for 8 minutes. PCR products were purified by QIAquick PCR Purification Kit (Qiagen, Hilden, Germany) or by 0.8% agarose gel electrophoresis followed by extraction with QIAquick Gel Extraction Kit (Qiagen, Hilden, Germany). Purified PCR products were sequenced (GATC-Biotech, Konstanz, Germany) using PCR primers and analyzed using BLASTn search (Zhang et al. 2000).