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Nanocomposite Membranes in Water Treatment
Published in P.K. Tewari, Advanced Water Technologies, 2020
Aquaporins are the protein channels that control water flux across biological membranes.279 Aquaporin is found widely in human tissues for rapid passive transport of water across cell membranes. Such transport channels exist in the cells of species in all three domains of life. Water movement in aquaporins is mediated by selective rapid diffusion caused by osmotic gradients.280 The hourglass shape of aquaporin-1 (AQP1), with selective extracellular and intracellular vestibules at each end, allows water molecules to pass rapidly in single file, while excluding proteins.281 A constant number of molecules are assumed to occupy the aquaporin channel at all times and the water molecules are assumed to move together in discrete translocations, or hops.282
Characterization of surface modified glycerol/silk fibroin film for application to corneal endothelial cell regeneration
Published in Journal of Biomaterials Science, Polymer Edition, 2019
Jeong Eun Song, Bo Ra Sim, Yoo Shin Jeon, Han Sol Kim, Eun Yeong Shin, Cristiano Carlomagno, Gilson Khang
To analyze the gene expression, Reverse-Transcription Polymerase Chain Reaction (RT-PCR) was performed using genes associated with rCEnC activity. Chosen genes were Na+/K+-ATPase (NaK), Aquaporin-1 (AQ1) and chloride voltage-gated channel 3 (CLCN3). The mRNA expression levels of rCEnCs were measured after 4 days of culture. The total RNA was extracted from cells on each film using TRIzol reagent (Takara, Japan) according to the manufacturer’s instructions. Isolated RNA was quantified using an Eppendorf BioSpectrometer (Eppendorf, Germany) to standardize the samples RNA quantity. The RT-PCR steps were set as follows: denaturation for 30 sec at 95 °C; annealing for 30 sec at different temperatures, depending on the used primer; elongation step 1 min/kb at 72 °C. Amplified genes were separated by electrophoresis at 100 V on 1.0% agarose gel in 0.5% TAE buffer and visualized using EtBr.
Two Co(II) coordination polymers: treatment and nursing values on acute renal failure during pregnancy by regulating the expression of aquaporin
Published in Inorganic and Nano-Metal Chemistry, 2021
Gui-Yu Hu, Wei Chen, Guo-Wei Song, He-Cun Xiao, Ying Han
Aquaporin-1 is a large amount of channel protein expressed in renal proximal convoluted tubule epithelial cells. Its content changes significantly during acute kidney injury, and it also changes significantly during the intervention of renal protective drugs. So, after the treatment of the new compounds, the expression levels of the Aquaporin-1 were measured with real time RT-PCR. The results in Figure 5 suggested that compared with compound 2, compound 1 showed stronger inhibitory activity on the abnormal expression of the Aquaporin-1 on renal tubular endothelial cells.