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Value-Added Products and Bioactive Compounds from Fruit Wastes
Published in Megh R. Goyal, Arijit Nath, Rasul Hafiz Ansar Suleria, Plant-Based Functional Foods and Phytochemicals, 2021
Ranjay Kumar Thakur, Rahel Suchintita Das, Prashant K. Biswas, Mukesh Singh
Invertase has lower crystallinity level than sucrose, which helps in keeping products soft and fresh for long time [85]. Reddy et al. [142] studied banana waste as fermentation substrate for Aspergillus niger to produce cellulase [167]. Okafor et al. [119] investigated production of pectinolytic enzymes using Penicillium chrysogenum and Aspergillus niger. Penicillium chrysogenum produced pectinase at a level of 220.3 IU mg protein1 when pineapple peel was used as a substrate.
Reproductive Biotechnologies Applied to Artificial Insemination in Swine
Published in Juan Carlos Gardón, Katy Satué, Biotechnologies Applied to Animal Reproduction, 2020
Francisco Alberto García-Vázquez, Chiara Luongo, Gabriela Garrappa, Ernesto Rodríguez Tobón
Although results obtained by inseminating with liquid semen stored at 15–17 ºC are like those obtained in natural mating, the lifespan of spermatozoa is limited and can only be preserved in average for 5 days (Michael et al., 2008). Despite the existence of good quality boar extenders, studies of newly improved formulations are incessant. There is a growing interest in improved long-term extender composition to achieve optimal storage conditions (Vyt et al., 2004; Funahashi and Sano, 2005; Lee and Park, 2015a,b; Bielas et al., 2017). In this sense, an improved in sperm membrane protection was accomplished when the extender was flowed through a neodymium magnet (Lee and Park, 2015a,b). These results suggest that magnetized semen extender before dilution could increase the longevity of spermatozoa preservation. A novel long-term semen extender was developed by Brescani et al., which allowed to stored doses for 12 days at 17 ºC. Its formulation was based on the incorporation of new molecules, as the energy precursor, a disaccharide (sucrose) and an enzymatic agent (invertase) (Bresciani et al., 2017).
Carbohydrate and glycosylation disorders
Published in Steve Hannigan, Inherited Metabolic Diseases: A Guide to 100 Conditions, 2018
Treatment includes dietary management with a low-sucrose or sucrose-free diet. A starch-free or low-starch diet may also be advised in the early years of life. In addition, vitamin supplements may be recommended. Some of those afected may beneit from yeast-derived invertase, which exhibits sucrase activity and is taken orally with food. Commercial enzyme replacement therapy is available in the form of the orphan drug Sacrosidase (Sucraid®). This has been approved by the Food and Drug Administration in the USA.
Effective antigen delivery via dual entrapment in erythrocytes and autologous plasma beads
Published in Journal of Drug Targeting, 2018
Munazza Tamkeen Fatima, Ejaj Ahmad, Mehboob Hoque
The assay procedure described by Bernfeld [34] was followed for the quantitation of invertase. The assay mixture included 150 μL of 0.2 M sodium acetate buffer pH 4.9, 100 μL of suspending buffer (20 mM PBS, pH 7.4) containing invertase and 50 μL of 0.5 M sucrose. The samples were incubated for 10 min at 37 °C and the reaction was stopped by the addition of 200 μL of 0.5 M sodium phosphate, pH 7.0, followed by boiling in water bath for 5 min. After cooling, 1.0 ml of 1% (w/v) DNS was added, incubated for 5 min at room temperature, and boiled for another 5 min. Three millilitres of distilled water was added to the tubes and the samples were cooled and absorbance recorded at 540 nm. One unit of invertase is the amount that converts 1.0 μmole of sucrose to glucose and fructose per minute at 37 °C.
Glucosinolate-Enriched Fractions from Maca (Lepidium meyenii) Exert Myrosinase-Dependent Cytotoxic Effects against HepG2/C3A and HT29 Tumor Cell Lines
Published in Nutrition and Cancer, 2022
Raquely M. Lenzi, Luciano H. Campestrini, Simone C. Semprebon, Jonas A.R. Paschoal, Monique A.G. Silva, Selma F. Zawadzki-Baggio, Mário S. Mantovani, Carmen L.O. Petkowicz, Juliana B.B. Maurer
The total phenolic content was determined using the Folin–Ciocalteu method (26) with GA as the standard. The results were expressed as µg GA equivalent (GAE)/mg fraction. Sucrose was detected using TLC. Further, the presence of sucrose was confirmed by treatment with invertase (5 min, 25 °C), followed by TLC analysis to confirm the disappearance of the substrate (sucrose) and the appearance of reaction products (Glc and Fru).