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Hysteroscopic Myomectomy
Published in Rooma Sinha, Arnold P. Advincula, Kurian Joseph, FIBROID UTERUS Surgical Challenges in Minimal Access Surgery, 2020
The hysteroscopic tissue removal system (TRS) for the treatment of SM myomas is usually applied with the patient under general or spinal anesthesia, typically as a day-case procedure. A specially designed TRS is introduced via the hysteroscope and used to cut and simultaneously aspirate the leiomyoma tissue. The aspirated tissue can be collected for histological analysis. Currently, there are three different kinds of hysteroscopic TRS:Truclear (Medtronic): The first to be approved by the US Food and Drug Administration (2005). The Truclear 8.0 has a diameter of 8 mm and is introduced into the uterine cavity with a 9-mm rigid sheath. The Truclear 5.0 hysteroscopy system incorporates a 2.9-mm rotatory-style blade through a 5-mm, 0° hysteroscope.MyoSure (Hologic) 19 Fr or 6.25 mm. MyoSure is introduced into the uterus through a 6- or 7-mm, 0°, continuous flow hysteroscope.Biggati Shaver (Karl Storz) in both 19 Fr and 24 Fr.
Genitourinary malignancies
Published in Alexander Trevatt, Richard Boulton, Daren Francis, Nishanthan Mahesan, Take Charge! General Surgery and Urology, 2020
Davoud Khodatars, Sarah Y. W. Tang
Cystoscopy Patients with haematuria (see Chapter 30) will require flexible cystoscopy to rule out malignancy. It is a key investigation to assess tumour invasion and biopsies can be taken for histological analysis.
Orthopaedic Oncology
Published in Manoj Ramachandran, Tom Nunn, Basic Orthopaedic Sciences, 2018
Nimalan Maruthainar, Rej Bhumbra, Steve Cannon
Tissue may be obtained for histological analysis by percutaneous (fine-needle/core-needle), or open (incisional/excisional) biopsy techniques. The technique utilized is based on tumour location, size, depth, biology, clinical resources and histological expertise within the unit.
Oxford’s clinical experience in the development of high intensity focused ultrasound therapy
Published in International Journal of Hyperthermia, 2021
Ishika Prachee, Feng Wu, David Cranston
A further study was designed as a part of these trials to test the ability of MRI to predict zones of necrosis following HIFU treatment [15,17]. Six patients with liver tumours were recruited in which tumour resection was conducted post-HIFU treatment. This allowed for histological analysis alongside the imaging results. Contrast-enhanced MRI successfully predicted complete ablation in three cases. In one case, the region of ablation on MRI seemed smaller than predicted at the time of HIFU, but histology showed complete ablation. The final two cases showed incomplete ablation. Furthermore, the estimated intra-operative ablation through B-mode ultrasound showed a clear correlation with the post-operative MRI estimates. In order to evaluate the intra-operative and MRI assessments of ablation, a slope of best fit was determined using linear regression. Results showed a slope of linear regression of 1.23 (95% CI = 0.68–1.77, p < 0.0001), indicating a clear correlation. HIFU-ablated tissue demonstrated coagulative necrosis as well as heat-fixation. However, the heat-fixed cells appeared normal under haematoxylin and eosin staining. Hence, this staining method is not a reliable indicator for HIFU-induced tissue death.
Association of upper and lower airway eosinophilic inflammation with response to omalizumab in patients with severe asthma
Published in Journal of Asthma, 2020
Makoto Kurokawa, Toshiyuki Koya, Hiroyuki Takeuchi, Masachika Hayashi, Takuro Sakagami, Kojiro Ishioka, Yasuhiro Gon, Takashi Hasegawa, Toshiaki Kikuchi
Nasal mucosa samples were fixed in 10% formalin and immersed in paraffin. Each section was cut to a thickness of 5 µm, deparaffinized, and stained with hematoxylin and eosin (HE) staining for histological analysis. Immunohistochemical staining was achieved by using antibodies of cluster of differentiation (CD)3 (clone: EP449E), CD20 (clone: L26), tryptase (clone: AA1), FcεRI (clone: CRA1), IgE (goat polyclonal), and eosinophilic major basic protein (MBP; clone: BMK) according to the manufacturer’s instructions. An antirabbit immunoglobulin G (IgG)-horseradish peroxidase-linked F(ab′)2 fragment (Amersham Pharmacia Biotech, Little Chalfont, UK) was used as the secondary antibody. After visualization with 3,3′-diaminobenzidine (Dojin Chemical, Kumamoto, Japan), the slides were counterstained with HE. Histological analyses were conducted as previously described [15]. Positive cells were counted in five randomly selected fields at 400× magnification and expressed as the average of cell numbers in each of the fields.
Universal existence of fluorescent carbon dots in beer and assessment of their potential toxicity
Published in Nanotoxicology, 2019
Haitao Wang, Shan Liu, Yukun Song, Bei-Wei Zhu, Mingqian Tan
Male BALB/c mice (18–22g) were purchased from Dalian Medical University. Approved protocols from the Institutional Animal Care and Use Committee at Dalian Polytechnic University were followed for all animal procedures. The mice were given 2 g kg−1 of CDs from Snow beer by a single oral administration to evaluate their biodistribution. The control group mice were orally administered a 0.9% NaCl solution. Mice were sacrificed at 0.5, 2, 6, 24 h after the CDs administration. The major organs were collected and imaged on a Multi-functional in vivo imaging system (Molecular Devices, San Jose, CA). The fluorescent intensity of organs under the excitation of 365 nm wavelength was recorded and shown as pseudocolor image. The fluorescence intensity was visualized by a different color. White-red indicates high fluorescence intensity, whereas blue-purple represents a weak intensity. Then these organ’s tissues were sectioned into slices for histological analysis. For acute oral toxicity investigate, mice were sacrificed on day 1 post-exposure. The control group mice were orally administrated a 0.9% NaCl and glucose-CDs of 2 g kg−1 body weight. The physiological and biochemical indexes were measured by using ADVIA 2400 Chemistry System (Siemens, Munich, Germany). Hematoxylin–eosin (H–E) staining of the major organs/tissue was carried out following the standard protocol.