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Tissue Engineering in Reconstruction and Regeneration of Visceral Organs
Published in Rajesh K. Kesharwani, Raj K. Keservani, Anil K. Sharma, Tissue Engineering, 2022
Soma Mondal Ghorai, Sudhanshu Mishra
Though the literature for lung TE is scarce, the possibility to use autologous or allogeneic lung cells expanded in vitro can be considered for future endeavors. Human embryonic stem cells (hESCs) or other stem cell-based approaches hold much promise in the creation of a respiratory device for preterm infants (Samadikuchaksaraei et al., 2006). This process follows immediate retrieval of the umbilical vein and umbilical artery of a preterm infant and instantly cannulated, so that these vessels can supply blood to the tissue-engineered scaffold of the lung. The scaffold is developed into an endothelialized vascular network, so blood could flow through it avoiding anticoagulation. Therefore, the field of TE of lung has the potential to provide a potent solution for future clinical applications.
Polymeric Nanoparticles for Drug Delivery
Published in Severian Dumitriu, Valentin Popa, Polymeric Biomaterials, 2020
Karine Andrieux, Julien Nicolas, Laurence Moine, Gillian Barratt
A nonselective selectin ligand (a modified sugar) was synthesized and coupled to a PLA homo-polymer bearing pendant carboxyl groups and rhodamine as a fluorescent tag by Banquy et al. (2008). Nanospheres prepared from these polymers bound to activated human umbilical vein endothelial cells that expressed both E- and P-selectin. Recently, in our laboratory, amphiphilic copolymers based on d,l-lactide and PEG macromonomer as the hydrophilic part have been prepared and decorated with glucose as a model sugar by click chemistry (Jubeli et al., 2010). These polymers form small nanospheres by nanoprecipitation, exposing the sugar at the surface. The ultimate aim is to attach sialyl LewisX, the physiological ligand for E-selectin, in order to target activated endothelium for the treatment of rheumatoid arthritis.
Engineering Biomimetic Scaffolds
Published in Claudio Migliaresi, Antonella Motta, Scaffolds for Tissue Engineering, 2014
Nasim Annabi, Nihal Engin Vrana, Pinar Zorlutuna, Fariba Dehghani, Ali Khademhosseini
Electrospinning has been applied for the fabrication of nanofibrous biomimetic scaffolds from natural polymers including chitosan,29 collagen,28 and elastin30,31; synthetic biodegradable polymers such as polyglycolic acid (PGA),32 poly(lactic-co-glycolic acid) (PLGA),33 poly(L-lactide) (PLLA), polycaprolactone (PCL),34 and poly(glycerol sebacate) (PGS)35; and combinations of natural and synthetic polymers. These scaffolds were used for the formation of a variety of tissues such as bone and cardiac muscles. In one study, electrospinning technique was used to fabricate highly porous nano-and microfiber mats fabricated from poly(L-lactide-co-caprolactone) (PLCL) co-polymers with different compositions.36 This polymer was used for the fabrication of fibers with diameters ranging from 0.3 mm to 7 mm and porosity between 56% and 63% (Fig. 7.1A-C). The constructs were then seeded with human umbilical vein endothelial
VEGF loaded porcine decellularized adipose tissue derived hydrogel could enhance angiogenesis in vitro and in vivo
Published in Journal of Biomaterials Science, Polymer Edition, 2022
Kaituo Liu, Ming Zhao, Yan Li, Liang Luo, Dahai Hu
Female porcine subcutaneous adipose tissues were purchased from local slaughterhouse and processed immediately after arriving laboratory, all time from purchase to process was no longer than 4 h. Unless stated otherwise, all chemical agents were purchased from Sigma-Aldrich (China). Cell counting Kit-8 (CCK-8) was purchased from Bimake (Shanghai, China), ELISA for VEGF was from Boster (Wuhan, China). Calcein-AM/PI double stain kit was from Dojindo (Shanghai, China). EdU 555 stain kit was from Beyotime (Shanghai, China). All primary antibodies (F-actin, CD31) and fluorescence secondary antibodies (Alex Fluor 594) were purchased from Abcam (China). Human Umbilical Vein Endothelial Cells (HUVECs) were purchased from GuangZhou Jennio Biotech Co. Ltd (GuangZhou, China), HUVECs without specific instruction were cultured with Endothelial Cell Medium (Sciencecell, China) supplemented with 5% FBS, 1% penicillin and 1% streptomycin at 37 °C and 5% CO2. All male 6-8weeks Balb/c mice utilized in in vivo experiments were purchased from the Experimental Animal Center of Fourth Military Medical University, and the mice were maintained on a 12 h light cycle. Animal experiments were approved by the Animal Experimental Ethics Committee of Fourth Military Medical University and conformed to the guidelines of the Committee. (Ethical number for Balb/c mice: XJYYLL-2015204)
A comparison between the mechanical properties of the hepatic round ligament and the portal vein: a clinical implication on surgical reconstruction of the portal and superior mesenteric veins
Published in Computer Methods in Biomechanics and Biomedical Engineering, 2020
Wentao Zhu, Rongqiang Song, Xuefeng Cao, Lei Zhou, Qiang Wei, Haibin Ji, Rongzhan Fu
Hepatic round ligament (HRL), also known as ligamentum teres, is the remnant of the embryonic umbilical vein, which degenerates after birth (Emre et al. 1993). It is located between the umbilicus and the left branch of the portal vein (PV), connecting the left hepatic vein or the inferior vena cava via the venous ligament. Anatomically, it can be divided into intraperitoneal and extraperitoneal segments. Structurally, it is organized into the inner, middle and outer layers and still retains the structural features of the blood vessel wall that is composed of collagen and elastic fibers, as well as smooth muscles. A distinct elastic muscle band enriched with smooth muscle, elastic and collagen fibers exist between the inner and middle layer. Blood supply to the HRL is sufficiently provided by the right hepatic artery and the umbilical vein. Clinically, narrowed or obstructed HRL can be widened to reconnect with the PV (Ikegami et al. 2008).
Facile synthesis of 3D silk fibroin scaffolds with tunable properties for regenerative medicine
Published in Journal of Biomaterials Science, Polymer Edition, 2020
Haiying Gao, Chenghui Huang, Youqi Zhu, Xilan Ma, Chuanbao Cao
ECV304 (human umbilical vein endothelial cells), one of the blood vessel related cells, was adopted to evaluate the tendency of vascular tissue differentiation [28]. For cell seeding, SF/HEP scaffolds infiltrated with FBS (fetal bovine serum) for 2 h to ensure that scaffolds adhered the bottom of well. Then ECV304 were seeded in 24-well tissue culture plate (1 × 104 cells/well) and cultivated at time (3D, 5D) at 37 °C in a 5% CO2 atmosphere [29]. The growth morphology of cells was observed with an optical microscope (IX71, Olympus, Japan). Cell viability was measured by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) assay as previous described [30,31]. The microplate reader (680, BIO-RAD, American) measured the absorbance of S1 and S2 at 570 nm with reference wavelength of 630 nm. The cell viability value calculated as follow Equation (3):