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Modern Microscopic Methods of Bioaerosol Analysis
Published in Christopher S. Cox, Christopher M. Wathes, Bioaerosols Handbook, 2020
The transmission electron microscope has provided a wealth of information on the ultrastructure of cells. It has a resolution limit of about 1 nm. Ultrathin sections must be prepared, which are stained with metals to provide adequate contrast. These metals may be conjugated onto antibodies for specimen identification. Again, owing to the use of a vacuum, living material cannot be examined. The system is expensive. Both scanning and transmission electron microscopes can be used for semi-quantitative elemental analysis when an X-ray microprobe analyzer is incorporated.
Kidney Structure and Physiology
Published in Joseph D. Bronzino, Donald R. Peterson, Biomedical Engineering Fundamentals, 2019
Joel M. Henderson and Mostafa Belghasem
2.3b and c). Transmission electron microscopy is particularly valuable for evaluating the ultrastructure of the glomerulus, as features of a variety of disease processes aecting the glomerulus may only be condently distinguished by examination at the ultrastructural level.
Static electric field inhibits the proliferation and secretion functions of splenic lymphocytes in mice
Published in International Journal of Environmental Health Research, 2023
Li Dong, Jiahong Wu, Guoqing Di
In the observation of cell ultrastructure, this study specifically referred to the observation method of Di et al. (2021). The spleen with a volume of 1 mm3 was obtained and was fixed in 2.5% glutaraldehyde. After 48 h, PBS was used to rinse the spleen. Next, the spleen was fixed with 1% osmic acid solution and rinsed with double distilled water. After that, 2% uranium acetate supernatant was used to fix and stain the spleen. Ethanol was used to dehydrate the spleen. The spleen was dehydrated with 100% acetone. At room temperature, the spleen was infiltrated with an embedding agent and pure acetone. The spleen was embedded with a pure embedding agent and placed in an oven. Finally, the spleen was cut into ultra-thin sections and stained with uranium acetate dye and lead citrate dye. A transmission electron microscope (TEM, Philips Tecnai 10, Netherlands) was used to observe the ultrastructure of cells.