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Methods for the Determination of Endocrine Disrupters
Published in Jason W. Birkett, John N. Lester, Endocrine Disrupters in Wastewater and Sludge Treatment Processes, 2002
The breast cancer cell line MCF-7 expresses the estrogen receptor (hERa), the glucocorticoid receptor (GR), and progesterone receptor (PR). Similar constructs have been introduced into this cell line,46 and this has become a routine method for measuring ER transcriptional activation.14 The cells may be transiently transfected, although permanent cell lines, such as the MVLN/MCF-7 have been developed.47 Similarly, other stable transfected cell lines have been developed from the MCF-7, the MELN, and MMLN.48 In addition to identifying estrogenic activity,49 these lines have also been used to investigate activity of PCB77, arochlor 1254, DDE, and atrazine relative to the progestin receptor agonist R5020 and androgen receptor agonist R1881. All these compounds were observed to act as antagonists to both the AR and PR; however, no antiglucocorticiod activity was observed.50
A novel gold nanorods-based pH-sensitive thiol-ended triblock copolymer for chemo-photothermo therapy of cancer cells
Published in Journal of Biomaterials Science, Polymer Edition, 2019
Somayyeh Fallah iri sofla, Mojtaba Abbasian, Mortaza Mirzaei
The in-vitro cell cytotoxic effects of samples on MCF7 cell was evaluated by MTT assay. Firstly, the cells were seed in a 96-wells plate with a cell density of 5 × 10 3 cells per well, subsequently, the cells were incubated and allowed to grow for 24 h. To assess the cytotoxicity of samples, the cells were treated with various concentrations of AuNRs-CTAB, AuNRs@polymer, and polymer. The cells were incubated for 48 h, then the medium was replaced with 100 μL fresh medium and 20 μL MTT solution (5 mg mL−1) subsequently, the cells were incubated with shaker incubator at the dark condition for 4 h. After incubation, the medium in each well was taken out and 150 μL DMSO was added to each well to solve him the formazan crystals.
Enhanced anticancer potency by thermo/pH-responsive PCL-based magnetic nanoparticles
Published in Journal of Biomaterials Science, Polymer Edition, 2018
Samad Hosseini Sadr, Soodabeh Davaran, Effat Alizadeh, Roya Salehi, Ali Ramazani
The cytotoxicity of produced blank nanoparticles and anti tumoral efficacy of dual anticancer drug loaded nanoformulation was evaluated by MTT assay. MCF7 cells (Human breast adenocarcinoma) were selected as a target cells and were created from Pasteur Institute Cell bank (Iran, Tehran). The cells were cultured in RPMI1640 medium including 10% fetal bovine serum, 100 mg/mL streptomycin and 100 IU/mL penicillin and incubated at 37 °C in an atmosphere of 5% CO2 and humidity more than 95%. For cell feeding, culture medium was replaced every day. They were separated with 0.25% trypsin in PBS (phosphate buffer solution, pH = 7.4). After separation, the cells were planted into 96-well plates at density of 2 × 104 cell/well and were incubated for 24 h at 37 °C in moist air with 5% CO2. After extraction and isolation of previous solution, different concentrations (25, 50, 100 and 200 μg/mL) of combined DOX and MTX at nano-formulated and free forms as well as Fe3O4@PCL grafted-poly (HEMA-co-MAA-co-NIPAAm-co-TMSPM) nanocomposites of different concentrations (250, 500 and 1000 μg/mL) along with new culture medium were added to specified wells. Control cells were subtending with equal volume of fresh media. The plates were incubated for 48 and 72 h. To execute the MTT assay, the old solution of cells was replaced with new solution which contains 200 mL of fresh medium and 50 mL of a 5 mg/mL MTT solution. After incubating for 4 h under cell growing conditions, the prior medium was vacated. To solve the produced formazan crystals, 100 μL DMSO was added and the absorbance of each sample was measured at 570 nm with a background correction at 630 nm.
Biological response of Schiff base metal complexes incorporating amino acids – a short review
Published in Journal of Coordination Chemistry, 2020
Alagarraj Arunadevi, Natarajan Raman
Mahmoud et al. reported a new Schiff base ligand having 2-acetylferrocene and L-histidine and a series of Cr(III), Mn(II), Fe(III), Co(II), Ni(II), Cu(II), Zn(II) and Cd(II) complexes [30]. The anticancer activities of these complexes on MCF-7 (Michigan Cancer Foundation-7) cell lines have been explored. It is known that cell lines seem to be a key element for the molecular diagnosis in breast cancer as they can be widely used in many aspects of laboratory research and, particularly, as in vitro models in cancer research. For breast cancer, MCF-7 cells represent very important candidates as they are used ubiquitously in research for estrogen receptor (ER)-positive breast cancer cell experiments and many sub-clones, which have been established, represent different classes of ER-positive tumors with varying nuclear receptor expression levels. MCF-7 cells are well-suited for anti-hormone therapy resistance studies since they are easily cultured and retain ER expression when they were treated with such targeted-therapy. Mahmoud et al. proved that the free ligand and Cu(II), Zn(II) and Cd(II) complexes have inhibitory activity against MCF-7. The Cd(II) complex shows potent activity with low IC50 value, while the activity of the ligand is greater than the Cu(II) and Zn(II) complexes, due to the nanosize of ligand and Cd(II) complex which increases their anticancer activities by decreasing their IC50 values whereas all other Schiff base metal complexes are inactive against MCF-7 cancer cell lines. The nanomatric structure of ligand and Cd(II) complex was confirmed by SEM analysis. The anticancer activities of the free Schiff base and its Cu(II), Zn(II) and Cd(II) complexes against MCF-7 are given in Table 3. The Schiff base and its metal complexes have also been tested on HBF4 (human normal melanocyte cell line) at high concentration (100 µg ml−1). The Cd(II) complex has surviving fraction of 55% at high concentration and has been recommended as a potent antibreast cancer drug, especially in very small concentrations (IC50 value = 3.5 µg ml−1) such that its toxic effect will not be apparent.