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Stem Cell Banking for Regenerative and Personalized Medicine
Published in Shaker A. Mousa, Raj Bawa, Gerald F. Audette, The Road from Nanomedicine to Precision Medicine, 2020
All adipose samples should be obtained with written consent from the donors and according to any other requirements of the local Institution Review Board (IRB). Adipose tissue samples are generally obtained from scheduled liposuction procedures, or by syringe harvest performed under local anesthesia. The lipoaspirates should be processed and cryopreserved within 24–36 h of collection. For cryopreservation, the tissue is washed extensively with isotonic saline, and the washed tissue slurry is directly placed in a cryo-container (generally a cryobag) and an equal volume of pre-cooled DMSO solution (70% Lactated Ringer’s buffer, 20% serum or HSA, 20% DMSO) is added slowly over several minutes at 4°C. The cryo-container is generally mixed at 4°C for 20–30 min to allow for cryoprotectant equilibration. Cryopreservation is performed using a controlled rate freezer to −180°C before final submersion in liquid nitrogen for long term storage [71]. In our experience we have not seen significant differences in AT-MSC harvest by tumescent liposuction, VASER liposuction, power-assisted liposuction or various forms of laser liposuction (although we have not analyzed all possible permutations that are commercially available). In addition, we have not observed any deleterious effects of anesthetic choice on the clinical utility of the harvested AT-MSC (i.e., Lidocaine and Marcaine were equivalent).
Stem Cell Banking for Regenerative and Personalized Medicine
Published in Shaker A. Mousa, Raj Bawa, Gerald F. Audette, The Road from Nanomedicine to Precision Medicine, 2019
All adipose samples should be obtained with written consent from the donors and according to any other requirements of the local Institution Review Board (IRB). Adipose tissue samples are generally obtained from scheduled liposuction procedures, or by syringe harvest performed under local anesthesia. The lipoaspirates should be processed and cryopreserved within 24–36 h of collection. For cryopreservation, the tissue is washed extensively with isotonic saline, and the washed tissue slurry is directly placed in a cryo-container (generally a cryobag) and an equal volume of pre-cooled DMSO solution (70% Lactated Ringer’s buffer, 20% serum or HSA, 20% DMSO) is added slowly over several minutes at 4°C. The cryo-container is generally mixed at 4°C for 20–30 min to allow for cryoprotectant equilibration. Cryopreservation is performed using a controlled rate freezer to −180°C before final submersion in liquid nitrogen for long term storage [71]. In our experience we have not seen significant differences in AT-MSC harvest by tumescent liposuction, VASER liposuction, power-assisted liposuction or various forms of laser liposuction (although we have not analyzed all possible permutations that are commercially available). In addition, we have not observed any deleterious effects of anesthetic choice on the clinical utility of the harvested AT-MSC (i.e., Lidocaine and Marcaine were equivalent).
Injection Materials for the Larynx
Published in Gilson Khang, Handbook of Intelligent Scaffolds for Tissue Engineering and Regenerative Medicine, 2017
Dong Wook Kim, Seong Keun Kwon
Autologous fat is regarded a durable long-term injection material because of its excellent biocompatibility. Several studies have demonstrated that lipoinjection has long-lasting effects with functional outcomes that are comparable to those of framework surgeries such as medialization thyroplasty.44,45 However, unlike injection laryngoplasty with CaHA, which can be easily performed with a small bore needle in an outpatient clinic, injection laryngoplasty with autologous fat requires an accompanying liposuction procedure, and the injection should be performed with a large-bore (18-gauge) needle under general anesthesia in the operating room. Another major problem with autologous fat injection is the unpredictable rate of resorption. It is reported that 30% of the injected fat is absorbed within one month, and it is completely resorbed in 5 months.46,47 To compensate for the early resorption, the lipoinjection procedure should be performed with the goal of substantial overinjection. However, excessive injection of fatty tissue into the vocal fold can result in poor voice quality and respiratory distress.
Numerical investigation of multi-pulsed cryogen spray cooling for skin cold protection in laser lipolysis
Published in Numerical Heat Transfer, Part A: Applications, 2020
Hui Xin, Bin Chen, Zhifu Zhou, Dong Li, Jiameng Tian
Overweight and obesity can induce severe chronic diseases, such as hypertension, diabetes, angiocardiopathy, cancer, and dyslipidemias [1, 2]. However, physical exercise and dietetic therapy are often unsatisfying in localized adiposity control. Tumescent liposuction is established to reshape body [3], although liposuction may cause complications, such as bacterial infection, permanent sensory nerve injury, and even death due to bleeding [4]. By contrast, noninvasive or minimally invasive laser lipolysis has prominent advantages in the treatment of obesity [5, 6], which can effectively promote fat dissolution; reduce ecchymosis, edema, and discomfort; facilitate local small vessel coagulation; boost collagen regeneration; and improve skin elasticity.