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EEMS2015 organizing committee
Published in Yeping Wang, Jianhua Zhao, Advances in Energy, Environment and Materials Science, 2018
LB medium: peptone 10 g, yeast extract 5 g, NaCl 10 g, distilled water 10 ml, pH 7.0. Liquid culture medium was added with agar (the concen- tration of 20 g/L) to prepare LB solid medium. LB culture medium is used for the amplification and cultivation of the purified strain.
Isolation and genomic characterization of Klebsiella Lw3 with polychlorinated biphenyl degradability
Published in Environmental Technology, 2022
Di-Hua Zhu, Fang-Hong Nie, Qing-Lang Song, Wan Wei, Min Zhang, Yao Hu, Hong-Ying Lin, Dan-Ju Kang, Zhi-Bao Chen, Jin-Jun Chen
Acetone, n-hexane, absolute ethanol, and BP were purchased from Macklin Biochemical Technology Co., Ltd. Chromatographically pure n-hexane, 99.93% PCB3 (4-Chlorobiphenyl) standard, and 98% PCB3 (4-Chlorobiphenyl) were purchased from J&K Bailingwei Technology Co. The mineral salt medium (MM30) comprised the following: (NH4)2SO4, 1 g; KH2PO4, 3 g; NaHPO4, 6.0 g; and trace metal salt solution, 0.5 mL. The trace metal salt solution contained the following: EDTA, 0.5 g; CaCO3, 1 g; FeSO4·7H2O, 0.5 g; MgSO4·7 H2O, 0.5 g; MnSO4·H2O, 10 g; Mixture44, 10 mL. Mixture44 contained the following: Na2B4O7·10H2O, 17.7 mg; CuSO4·5H2O, 39.2 mg; CaCl2·5H2O, 20.1 mg; ZnSO4·7H2O, 10.95 mg; and EDTA, 250 mg, in 1 L of double distilled water (pH 7.0). Solid MM30 was prepared by adding 2% agar to MM30 medium. After autoclaving the medium at 121 °C and 0.1 MPa for 20 min, biphenyls (aseptic) were added the sole carbon source. The LB intermediary medium was composed of the following (g/L): NaCl, 10.0; yeast extract, 5.0; and tryptone 10.0, pH 7.0 (121°C, 0.1 MPa, 20 min autoclaving).
Cloning, expression and characterization of a HER2-alpha luffin fusion protein in Escherichia coli
Published in Preparative Biochemistry and Biotechnology, 2019
Farzaneh Barkhordari, Nooshin Sohrabi, Fatemeh Davami, Fereidoun Mahboudi, Yeganeh Talebkhan Garoosi
In batch culture systems, LB is the medium which is used routinely. Although it is a common bacterial source of carbon and energy but it does not support high cellular mass formation and it is relatively costly. On the other hand, TB medium is more appropriate for obtaining high cell densities with lower cost.[42] Finding out the least and the best concentration of inducer is another important issue in optimization of E. coli protein expression in T7 based systems.[43] Studies have shown that higher IPTG concentrations may have toxic effects on the host with decreased cellular growth rate and increased amounts of low quality protein expression in the form of inclusion bodies.[44,45] In the present study, the highest expression level was achieved using 0.2 mM IPTG in both high producer E. coli strains (BL21 DE3 and pLysS) in TB medium reconfirming previous studies.
Isolation and characterization of phenol degrading bacterium strain Bacillus thuringiensis J20 from olive waste in Palestine
Published in Journal of Environmental Science and Health, Part A, 2018
Suheir I. Ereqat, Ahmad A. Abdelkader, Abedelmajeed F. Nasereddin, Amer O. Al-Jawabreh, Taher M. Zaid, Ilya Letnik, Ziad A. Abdeen
OMWW and solid olive mill waste (SOMW) samples were collected in October 2015 from an olive mill in Bethlehem, south of Palestine. All samples were collected in sterile containers and delivered to the laboratory within 24 h. Two types of growth media were used in the present study: The minimal salt media (MSM) and Luria-Bertani (LB). The LB, a nutritionally- rich medium, which is primarily used for the growth of bacteria contained 10 g tryptone, 5 g yeast extract and 10 g NaCl in 1,000 mL double-distilled water. The MSM contained Na2HPO4 (6 g), KH2PO4 (3 g), NaCl (0.5 g), NH4Cl (1 g), CaCl2·2H2O (0.02 g) and MgSO47·H2O (0.5 g) in 1,000 mL double distilled water.