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Medium Design for Cell Culture Processing
Published in Wei-Shou Hu, Cell Culture Bioprocess Engineering, 2020
A chemically defined medium contains only components whose chemical composition is known and characterized, and has all of its chemical species specified (Panel 7.6). It does not contain any mixture of components with an unknown or undefined composition. For example, “lipids” or “phospholipids” are not well-defined compounds, but are mixtures of a class of compounds that are not chemically specified. A chemically defined medium may contain purified growth factors, cytokines, and carrier proteins. A chemically defined medium is not necessarily protein-free.
Effects of various pine needle extracts on Chinese hamster ovary cell growth and monoclonal antibody quality
Published in Preparative Biochemistry & Biotechnology, 2023
Dingyue Zhang, Jinshu Qiu, Qing-Tian Niu, Tingting Liu, Rulin Gu, Xiaoying Zhang, Shun Luo
Dimethyl sulfoxide (DMSO) and dextrose dry powder were purchased from Sigma-Aldrich (USA). Formic acid, methanol, acetonitrile, water, and trifluoroacetic acid (TFA) were ordered from Thermo Fisher Scientific (USA). Phenol concentrated sulfuric acid, sodium chloride, sodium hydroxide, phosphate-buffered saline (PBS), and aluminum chloride were purchased from Sinopharm Chemical Reagent Co., Ltd., China. Rutin standard was ordered from Chengdu Nakeli Bio-Technology Corp. China. Chemically defined medium CHO 050, and supplement CD Feed were provided by Jianshun Biotechnology Co., Ltd., China, and the cell line used was a stable recombinant CHO K1 cell line provided by Thousand Oaks Biopharmaceuticals Corp., China. PNWE and PNEE were purchased from Shaanxi Sinuote Biotechnology Co., Ltd., China. PNPE was obtained from Lanzhou Wotelaisi Biotechnology Corp., China. PNWE and PNEE were extracted from the pine needle by water and 75% ethanol, respectively. PNPE were obtained from the pine needle by multi-step extraction with water. In PNPE 50% of the active ingredients are the polysaccharides according to the specification.
A comparative study of the bispecific monoclonal antibody, blinatumomab expression in CHO cells and E. coli
Published in Preparative Biochemistry and Biotechnology, 2018
Fatemeh Naddafi, Farshad H. Shirazi, Yeganeh Talebkhan, Maryam Tabarzad, Farzaneh Barkhordari, Zahra Aliabadi Farahani, Elham Bayat, Reza Moazzami, Fereidoun Mahboudi, Fatemeh Davami
CHO-S cells were cultivated in the ProCHO5 medium. The medium was supplemented with 4 mM l-glutamine, 2 mM PenSterp, and anticlumping agent. The cells were kept in a humidified incubator at 37 °C with 5% CO2 atmosphere. The cells were cultivated either in T-flasks or shaken in glass bottles. The cells were subcultured twice a week at a density of 3 × 105 cells/mL. Suspension CHO-DG44 host cell line was cultivated and transfected in a chemically defined medium (Life Technologies) using X-tremeGENE HP as a transfection vehicle. They were supplemented with 8 mM l-glutamine and 1% penicillin/streptomycin (100 μg/mL) in disposable vented cap flasks and at 37 °C in humidified atmosphere of 5% CO2. The cells were sub-cultured every three days at a density of 3 × 105 cells/mL. Trypan blue exclusion method was used to determine cell number and viability.