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Nanoemulsions: Status in Antimicrobial Therapy
Published in Adwitiya Sinha, Megha Rathi, Smart Healthcare Systems, 2019
Atinderpal Kaur, Rakhi Bansal, Sonal Gupta, Reema Gabrani, Shweta Dang
Respiratory infection is the major infection in patients suffering from cystic fibrosis. Among the various microorganisms responsible for respiratory infection, Burkholderia cepacia complex is the most harmful in cystic fibrosis (LiPuma, 2001), as it is among the most antimicrobial-resistant microorganisms. LiPuma et al. (2009) investigated the antibacterial activity of NE against 150 bacterial isolates recovered from cystic fibrosis (CF) respiratory tract specimens. The growth of all selected strains was inhibited by NE with minimum inhibitory concentration (MIC50) of 1.2 μg/mL and MIC90 of 125 μg/mL. NB-401 was more active against non-Burkholderia strains than Burkholderia strains. NE was thus effective in treating respiratory infections related to cystic fibrosis.
Burkholderia cepacia Complex (BCC) in Your Facility
Published in Jeanne Moldenhauer, Disinfection and Decontamination, 2018
However, since this organism is hard to differentiate from other similar organisms and in many cases the publications listed in this report either had the identification testing performed at the Burkholderia cepacia Research Laboratory (recognized worldwide as experts in BCC) and/or performed the testing including Burkholderia cepacia in the test panel, there is a concern as to whether the identification is correct or whether there is a possibility that this is actually Burkholderia cepacia. This is further complicated by another recent lot of product being contaminated with Burkholderia cepacia. Like Burkholderia cepacia this organism has been shown to have significant health concerns to individuals with cystic fibrosis.
Characterization of Burkholderia cepacia complex from environment influenced by human waste
Published in International Journal of Environmental Health Research, 2022
Jasna Hrenovic, Martina Seruga Music, Martina Drmic, Lucija Pesorda, Branka Bedenic
The Burkholderia cepacia complex (BCC) is a group of gram-negative nonfermentative bacteria that currently includes 21 closely related species (CLSI 2019; EUCAST 2021). The members of BCC were firstly described by Burkholder in 1950 as plant pathogen (Burkholder 1950). Further, the BCC were commonly reported in plant rhizosphere, soli and freshwater mud (Miller et al. 2002). From 1980s the BCC are more often reported as opportunistic pathogen in immunocompromised patients (Isles et al. 1984). The BCC, with B. multivorans and B. cenocepacia as predominant species, is known as a cause of chronic respiratory infection among patients suffering from cystic fibrosis (CF), but infect also the non-CF patients. Both sporadic infections and hospital outbreaks express a wide range of clinical manifestations (Mahenthiralingam et al. 2005).
Production of new lipase from Preussia africana and partial characterization
Published in Preparative Biochemistry & Biotechnology, 2022
Gustavo F. Oliveira, Milena R. L. Silva, Daniela B. Hirata
The process for the production of any enzyme involves the production and purification steps. Generally, the purification step contributes substantially to the overall cost of an enzyme. Some authors searched to integrate these steps in order to facilitate the downstream process. Sankaran et al.[31] reported the lipase production by Burkholderia cepacia, incorporating the fermentation and extraction processes using flotation system. Šibalić et al.[14] used a waste substrate for the lipase production from Thermomyces lanuginosus via solid-state cultivation and a vacuum-evaporation technique. The present study chose to study the preferential production of only one enzyme, thus reducing the purification steps at the end of the process.
Extractive fermentation for process integration of protease production by Aspergillus tamarii Kita UCP1279 and purification by PEG-Citrate Aqueous Two-Phase System
Published in Preparative Biochemistry & Biotechnology, 2022
Raniele Oliveira Alves, Rodrigo Lira de Oliveira, Osmar Soares da Silva, Ana Lúcia Figueiredo Porto, Camila Souza Porto, Tatiana Souza Porto
The enzyme was subjected to a variation in temperature, to verify their stability. The protease proved to be stable in the range of 30–60 °C during 180 min (Figure 4B). Sales et al.[16] in the integrated process of production and extraction of fibrinolytic protease from Bacillus sp. UFPEDA 485 observed that with increasing temperature the protease became more stable, maintaining 80% of its activity after 60 min of exposure to the optimum temperature. Show et al.[12] in the use of extractive fermentation for better production and recovery of lipase derived from Burkholderia cepacia using a thermo-separating polymer in an Aqueous Two-Phase System found that there was an improvement in the production of lipases with increasing temperature.