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Label the Patient’s Affect
Published in Scott A. Simpson, Anna K. McDowell, The Clinical Interview, 2019
Many people have difficulties putting their emotions into words, particularly when those emotions overwhelm one’s ability to think critically and rationally. An inability to describe one’s own emotions is called alexithymia; chronic alexithymia is seen among patients with severe depression, schizophrenia, developmental disabilities, and somatic symptom disorders.5 But often patients who are overwhelmed and highly emotional have momentary difficulty describing their emotions, too. Affect labeling helps patients verbalize their emotions and serves as a useful adjunct to empathy statements.
Tetraspanin-decorated extracellular vesicle-mimetics as a novel adaptable reference material
Published in Journal of Extracellular Vesicles, 2019
Estefanía Lozano-Andrés, Sten F. Libregts, Victor Toribio, Félix Royo, Sara Morales, Soraya López-Martín, Mar Valés-Gómez, Hugh T. Reyburn, Juan Manuel Falcón-Pérez, Marca H. Wauben, Manuel Soto, María Yáñez-Mó
To further characterise the presence of recombinant tetraspanins on the surface of these nanovesicles in their native state, cryo-EM was combined with immunogold labelling. To this end, an incubation step with specific antibodies directed against CD9 or CD63 was followed by incubation with secondary antibodies conjugated to 15 nm gold particles. Niosomes positively decorated with CD9 and CD63 were observed (Figure 5(b)), although the number of gold-particles associated to the niosome-surface was lower than expected. Due to the small size of niosomes steric hindrance interactions between antibodies (with an average size of around 10 nm and coupled to gold nanoparticles of 15 nm) are likely to affect labelling intensity. Another important consideration when performing immunolabelling is the chemical fixation process, which might alter the ultrastructure of samples [27].