China
Africa
Explore chapters and articles related to this topic
Breast Imaging with 99mTc-Methylenediphosphonate
Published in Raymond Taillefer, Iraj Khalkhali, Alan D. Waxman, Hans J. Biersack, Radionuclide Imaging of the Breast, 2021
Secondo Lastoria, Sergio Piccolo, Pietro Muto
The evaluation of the 99mTc-MDP uptake in human breast cancer cell lines is under investigation in our laboratory. 99mTc-MDP uptake was evaluated at the following time points: 1,5, 10, 40, 60, and 120 min either at 4 and 37°C. 99mTc pertechnetate was used as control. For these experiments human breast cancer cell line MCF-7 was used. The cells were detached from the flasks and initially resuspended in 0.5 mL of RPM1 1640 medium (500,000 cells/tube), centrifuged, and washed twice with phosphate-buffered saline (PBS). The pellets containing MCF-7 cells were then resuspended in 0.5 mL of PBS and incubated with 10 μCi in 10 (iL of 99mTc-pertechnetate and 99mTc-MDP at 4 and 37°C. After incubation the tubes were centrifuged and both pellet and supernatant were counted in a y counter. Duplicates were obtained for each experimental time point. 99mTc-MDP was concentrated in negligible amounts within human breast cancer cell lines MCF-7. Our preliminary data mirror the results of a previous study, recently published (24). However, the 99mTc-MDP cell-related activity was initially (1-10 min) higher (five- to seven-fold) than the 99mmTc-pertechnetate activity, suggesting that a very early, not stable binding to the cell membranes occurs. In fact, in none of our experiments, as well as in other studies, is 99mTc-MDP internalized within the cells as occurs for sestamibi (24).
Essential Oils in Cancer Therapy
Published in K. Hüsnü Can Başer, Gerhard Buchbauer, Handbook of Essential Oils, 2020
Carmen Trummer, Gerhard Buchbauer
Ortiz et al. (2016) examined the cytotoxic and genotoxic effects of sandalwood (Santalum album L. Santalaceae) EO. The EO which is obtained from trees from the Santalum genus is also used in the cosmetic, perfume, and food industries. For their studies, they used breast adenocarcinoma (MCF-7) and nontumorigenic breast epithelial (MCF-10 A) cells. (Z)-α-Santalol (25.3%) is one of the main components of the 300 identified chemical constituents of sandalwood EO. Other main active constituents were (Z)-nuciferol (18.3%), (E)-β-santalol (11.0%), and (E)-nuciferol (10.5%). The authors tested eight different concentrations of sandalwood EO and all showed a decrease in cell viability. The calculated IC50 value for the MCF-7 cell line was 8.03 μg/mL, and for the MCF-10A cell line, it was 12.3 mg/mL.
Estradiol-BSA Conjugates for Estrogen Receptor Localization: the Vienna Experience
Published in Louis P. Pertschuk, Sin Hang Lee, Localization of Putative Steroid Receptors, 2019
Maximilian Binder, Klaus Czerwenka, Manfred Boehm, Juergen Spona, Raimund Jakesz, Roland Kolb, Georg Reiner
For our experiments we used MCF-7 cells, an ER-positive human mammary carcinoma cell line, which was originally provided by the American Type Culture Collection. These cells were routinely grown in RPMI 1640 medium supplemented with 10% fetal bovine serum. For experimental studies this medium was replaced by medium containing charcoalstripped serum 72 hr prior to experiments. For incubation with E2 the culture medium was removed and replaced by a phosphate buffer containing CaCl, MgCl, and glucose (pH 7.3). E2 was added in 1000 x concentrated solutions in absolute ethanol. In addition, two other receptor-negative human mammary carcinoma cell lines derived from the pleural effusions of patients with metastatic breast cancer were included for control experiments (MR-GA and LJ-LH). These cells were maintained as described above for the MCF-7 cells. The receptor content of each cell line was determined weekly with the DCC assay. ER content of MCF-7 cells proved to be 47 fmol/mg protein (x = 47 + 11 SD, n = 10) with a Kd of 5.2 x 10-'°M.
Anticancer activity of linalool: comparative investigation of ultrastructural changes and apoptosis in breast cancer cells
Published in Ultrastructural Pathology, 2022
Hulya Elbe, Feral Ozturk, Gurkan Yigitturk, Tuba Baygar, Turker Cavusoglu
When the H-E staining of the control group of the MCF-7 cell line is examined, it is seen that the cells have epithelial morphology. In some areas, it is observed that the cells are concentrated and aggregated. In the linalool-treated group of the MCF-7 cell line, colony morphologies were observed to deteriorate due to a significant decrease in the number of cells (p < .001). Reduction in nuclear volume and hyperchromasia in the nuclear structure were commonly detected in cells (Figure 3). When the H-E staining of the control group belonging to the MDA-MB-231 cell line (no treatment was applied), round and spindle-shaped cell morphologies characterized by the cell line used was determined. Cell colonies were homogeneously dispersed at varying cell densities. In the linalool-treated group of the MDA-MB-231 cell line, the number of spindle-shaped cells decreased significantly compared to the control group (p < .001), while the number of round-shaped cells remained the same (p > .05). The decrease in the cytoplasm/nucleus volume ratio of the cells indicates the atrophic cell pattern. It was determined that the colony formation structures and the number of cells forming the colony were significantly decreased compared to the control group (p < .001) (Figure 4).
Novel daidzein molecules exhibited anti-prostate cancer activity through nuclear receptor ERβ modulation, in vitro and in vivo studies
Published in Journal of Chemotherapy, 2021
R. Ranjithkumar, K. Saravanan, B. Balaji, S. Hima, S. Sreeja, S. R. Timane, M. Ram Pravin Kumar, S. Kabilan, M. Ramanathan
The human breast cancer cell line MCF-7 expresses predominantly the ERα receptor and in the presence of estrogen or estrogen mimetic substances, MCF-7 gives a proliferation response. In comparison to binding assays, proliferation has the benefit of being a biological response which has been taken as a quantification of the direct interaction of agonists with the ER and equated with the estrogenic potential.19 Hence, in the present study, the stimulatory action of the modified daidzein derivatives in estrogen-sensitive MCF-7 human breast cancer cell line was tested in the presence or the absence of E2. The modified daidzein derivatives (1-7) at concentrations (1, 5, 10, and 25 µM) stimulated the growth of MCF-7 cancer cells. This was attributed to the non-genomic or ER independent mechanisms of genistein, which includes inhibition of tyrosine kinase and DNA topoisomerase activities, suppression of angiogenesis, arresting cell growth by interfering with signal transduction pathways and inhibiting the actions of cytokines and growth factors.33–37 Similarly, the NCEs in the present study may act through ER independent mechanisms to inhibit proliferation while E2 restored the proliferation of MCF-7 cells equal to control cells when treated with NCEs 6-8.
Exploiting ionisable nature of PEtOx-co-PEI to prepare pH sensitive, doxorubicin-loaded micelles
Published in Journal of Microencapsulation, 2020
Naile Ozturk, Asli Kara, Sevgi Gulyuz, Umut Ugur Ozkose, Mehmet Atilla Tasdelen, Asuman Bozkir, Ozgur Yilmaz, Imran Vural
MCF-7 cells were used to evaluate anticancer effect of DOX-loaded formulations. MCF-7 is a suitable model cell line for breast cancer research and anticancer drugs are often tested on this cell line (Lee et al.2015). MCF-7 cells are sensitive to DOX and half-maximal inhibitory concentration (IC50) of DOX solution was reported as 1.19 μM for MCF-7 cells at 72 h incubation period (Han et al.2012). Cells were seeded in 96-well plates at a density of 5 × 103 cells and incubated overnight. Then they were treated with blank micelles, DOX-loaded polymeric micelles, DOX solution, and plates were incubated for 24, 48, and 72 h at 37 °C. At the end of the incubation times, MTT solution (5 mg/mL) was added to wells and 4 h later 23% (w/v) SDS in 45% (v/v) DMF (pH 4.7) was added and plates were incubated overnight. The cell viability was analysed by absorption reading at 570 nm and 650 nm (reference wavelength) using a microplate reader (Versamax, Molecular Devices, San Jose, CA). The cell viability of treated wells was calculated by using absorption values of control wells as 100%.