China
Africa
Explore chapters and articles related to this topic
Bowel disorders
Published in Henry J. Woodford, Essential Geriatrics, 2022
A stool sample should be obtained when a recent onset of diarrhoea is reported to exclude an infective cause. A change in bowel habits raises the concern of an underlying malignancy. Colonoscopy should be considered, especially if there is associated weight loss or rectal bleeding. More detailed studies of anal structure and function, such as anal ultrasound and manometry or defecating proctograms, are usually unnecessary unless surgical intervention is being planned.
Infectious Diarrhoea
Published in Firza Alexander Gronthoud, Practical Clinical Microbiology and Infectious Diseases, 2020
Indications for sending stool sample to the laboratory: Type of stool: Severe, prolonged or bloody diarrhoeaClinical symptoms: Fever of ≥38.5°C, dehydration or systemic signs of infectionMedical history: Significant comorbidities, immunocompromised, recent use of antibiotics
Gastro-intestinal (digestive) system
Published in David Sales, Medical IELTS, 2020
A stool sample may need some clarification as the generic word sample is often interpreted as being a urine sample but an explanation as to the purpose of the test, such as faecal occult blood (FOB) (hidden blood in the stool) will be helpful.
Reproducible changes in the anorexia nervosa gut microbiota following inpatient therapy remain distinct from non-eating disorder controls
Published in Gut Microbes, 2022
Farnaz Fouladi, Emily C. Bulik-Sullivan, Elaine M. Glenny, Laura M. Thornton, Kylie K. Reed, Stephanie Thomas, Susan Kleiman, Ashlie Watters, Judy Oakes, Eun-Young Huh, Quyen Tang, Jintong Liu, Zorka Djukic, Lauren Harper, Yesel Trillo-Ordoñez, Shan Sun, Ivory Blakely, Philip S. Mehler, Anthony A. Fodor, Lisa M. Tarantino, Cynthia M. Bulik, Ian M. Carroll
Stool sample collection from patients with AN and non-ED controls has been previously described.13 Briefly, patients with AN provided a stool sample at admission to CEED or ACUTE (AD) and discharge from CEED or ACUTE after clinical renourishment (DIS). Non-ED controls provided a single stool sample. All samples were stored at 4°C until they were either transported (AN samples) or shipped overnight with ice packs (non-ED samples) to laboratories at UNC-CH or Denver Health. Upon arrival at the laboratory, fresh stool samples were immediately mechanically homogenized, aliquoted into 2-mL cryovials, and stored at −80°C for future microbiota characterization. Non-ED control samples were collected at home using a stool collection kit and stored at 4°C until they were shipped overnight with ice packs to our laboratory. Exposure of human stool samples to ambient air and extended periods at 4°C may influence the composition of the fecal microbiota. We attempted to limit these exposures to maintain the integrity of fecal microbial communities.
Evaluating live microbiota biobanking using an ex vivo microbiome assay and metaproteomics
Published in Gut Microbes, 2022
Xu Zhang, Krystal Walker, Janice Mayne, Leyuan Li, Zhibin Ning, Alain Stintzi, Daniel Figeys
The protocol for human stool sample collection (# 20160585–01 H) was approved by the Ottawa Health Science Network Research Ethics Board at the Ottawa Hospital, Ottawa, Canada. Four healthy volunteers (V31, V33, V34 and V51, with age of 34, 31, 48, and 49 years old, respectively; two men and two women) were recruited for stool sample collection. Exclusion criteria for participation included the presence of irritable bowel syndrome, inflammatory bowel disease, or diabetes diagnosis; antibiotic use or gastroenteritis episode in three months preceding collection; use of pro-/pre-biotic, laxative, or anti-diarrheal drugs in the last month preceding collection; or pregnancy. Approximately 8 g of fresh stools were collected from the volunteers and were immediately kept on dry ice or immersed in 20 mL pre-reduced deoxygenated preservation buffer in 50 mL sterile conical centrifuge tubes. The deoxygenated preservation buffer was prepared with sterile phosphate-buffered saline (PBS, pH 7.6) with a final concentration of 10% (v/v) glycerol and 0.1% (w/v) L-cysteine hydrochloride. Prior to usage, the preservation buffer was stored in anaerobic workstation (5% H2, 5% CO2, and 90% N2) for overnight with the lid open for gas exchange.
A case report of improvement on ADHD symptoms after fecal microbiota transplantation with gut microbiome profiling pre- and post-procedure
Published in Current Medical Research and Opinion, 2022
Suet Li Hooi, Jacky Dwiyanto, Haikel Rasiti, Kai Yee Toh, Reuben Kong Min Wong, Jonathan Wei Jie Lee
This case study was approved by AMILI Institutional Review Board (IRB) with reference number 2022/0401. The patient was informed of the study and publication plan and had provided explicit written consent. FMT donor was screened in accordance with the international consensus14–19. Fresh stool sample from the donor was then immediately packed in an airtight container under cool and anaerobic conditions and transported to the laboratory for further processing within four hours. Under anaerobic condition, the feces were homogenized in 0.9% saline solution, whereby the supernatant drained, and the pellet was resuspended with 15% glycerol and 0.9% normal saline. The patient then had three days of oral vancomycin, before undergoing a bowel cleansing and FMT delivery via colonoscopy.