China
Africa
Explore chapters and articles related to this topic
Host-Parasite Interactions With Macrophages In Culture
Published in Hans H. Gadebusch, Phagocytes and Cellular Immunity, 2020
Lee S. F. Soderberg, Morris Solotorovsky
The culture conditions that are most often used for macrophages have been heavily influenced by the culture system designed by Mishell and Dutton80 to demonstrate plaque-forming cells among mouse spleen cells. The culture medium generally consists of a balanced salt solution, such as Earle’s, Hank’s, or Tyrode’s, usually used in conjunction with a nutritional medium. Eagle’s minimal essential medium that was used by Mishell and Dutton80 is the nutritional medium reported most often in recent literature. Medium 199 and RPMI 1640 are also frequently used to culture macrophages. Other media that are used for macrophage culture include NCTC 109, Trowell’s T81, McCoy 5A, and Dulbecco’s.
Published in Ronald M. Atlas, James W. Snyder, Handbook Of Media for Clinical Microbiology, 2006
Ronald M. Atlas, James W. Snyder
Preparation of Medium: Aseptically combine 1.0L of sterile Eagle’s minimal essential medium with Hanks’ salts (MEMH) and 1.0L of sterile L 15 medium, modified Leibovitz. Mix thoroughly. Immediately prior to use, aseptically add 200.0mL of fetal calf serum and 50.0mL of NaHCO3 solution. Mix thoroughly. Aseptically distribute into sterile containers.
In vitro Anti-colorectal Cancer Potential of the Medicinal Mushroom Ganoderma neo-japonicum Imazeki in Hyperglycemic Condition: Impact on Oxidative Stress, Cell Cycle and Apoptosis
Published in Nutrition and Cancer, 2022
Meng-Fei Lau, Kek-Heng Chua, Vikineswary Sabaratnam, Umah Rani Kuppusamy
Dulbecco’s modified Eagle’s medium [DMEM: normal glucose (NG), 11885 Gibco; high glucose (HG), 11995 Gibco] and Eagle’s minimum essential medium (EMEM, D2279 Sigma) were supplemented as previously described (20). Methylthiazolyldiphenyl-tetrazolium bromide powder (MTT, 475989 Calbiochem), radioimmunoprecipitation assay lysis buffer (RIPA, 20-188 Millipore), Bradford reagent (B6916 Sigma), 4-amino-5-methylamino-2′,7′-difluorofluorescein diacetate (DAF-FM, D23844 Invitrogen), annexin V binding buffer (ABB, 556454 BD Pharmingen), annexin V-FITC (556419 BD Pharmingen), propidium iodide (PI, P4864 Sigma), and RNase A (12091021 Invitrogen) were purchased from respective manufacturers. Solvents (AR grade), namely absolute ethanol, hexane and chloroform were obtained from Fisher Scientific.
Nanocoating and biological evaluation of clindamycin- and rifampicin-loaded nanospheres impregnated silicone tube for antibacterial application
Published in Pharmaceutical Development and Technology, 2022
Watunyu Thanongsak, Atthaporn Boongird, Norased Nasongkla
Cytotoxicity was performed according to ISO 10993 standard by Laboratory for Biocompatibility Testing of Medical Devices, Department of Biomedical Engineering, Faculty of Engineering, Mahidol University. The protocol was derived from previous research (Srisang and Nasongkla 2019a). Silicone tubes were immersed in 1 mL of Eagle’s minimum essential medium (EMEM) for 24 h. The extractant concentration from silicone tubes was prepared at 25, 50, 75, and 100%. The extractant was incubated with L929 cells for 24 h (37 °C, 5% CO2) in 96 well plate. After removing the extracted media, cells were washed with PBS thrice. MTT solution was added into each well to evaluate cell viability. Then, MTT was washed out with DMSO. UV–vis spectroscopy was used to measure the percentage of cell viability at the absorbance of 570 nm.
In vitro exposure of human lens epithelial cells to X-rays at varied dose-rates leads to protein-level changes relevant to cataractogenesis
Published in International Journal of Radiation Biology, 2021
Vinita Chauhan, Ngoc Q. Vuong, Simran Bahia, Nazila Nazemof, Premkumari Kumarathasan
An HLE cell line that was transformed with SV-40 virus (CRL-11421; ATCC, Burlington, Canada) was cultured and maintained in an incubator at a temperature of 37 °C and under optimal humidity and atmospheric environment (5% CO2/95% air) as described by Bahia et al. (2018). The cells were grown in Eagles minimum essential medium (EMEM) containing 20% fetal bovine serum (FBS) (Sigma-Aldrich Canada, Oakville, ON, Canada) to confluence, to ensure that they were synchronized to the same phase of the cell cycle. Cells (1.0 × 106/60 mm dish) were seeded in media to a total of 3 mL in dishes 24 h prior to exposures. Immediately before experiments, the media of the cell was replenished with fresh media (3 ml) supplemented with 100 μg/mL of streptomycin and 100 units/mL of penicillin (Invitrogen, Canada Inc.).