China
Africa
Explore chapters and articles related to this topic
Mitochondrial encephalomyelopathy, lactic acidosis, and stroke-like episodes (MELAS)
Published in William L. Nyhan, Georg F. Hoffmann, Aida I. Al-Aqeel, Bruce A. Barshop, Atlas of Inherited Metabolic Diseases, 2020
William L. Nyhan, Georg F. Hoffmann, Aida I. Al-Aqeel, Bruce A. Barshop
Renal involvement may take the form of renal tubular acidosis, and there may be a typical renal Fanconi syndrome [39]. One patient developed a nephrotic syndrome and had focal glomerulosclerosis [16]. A variety of other organs have been involved in individual patients. One had pancreatitis following valproate administration [15]. Others have had peripheral neuropathy with or without rhabdomyolysis [40]. The histologic signature of the MELAS syndrome is the appearance of ragged red fibers in the muscle (Figure 51.8) [1, 12, 13, 37]. These are best seen in the trichrome stain. In H&E, there may be variation in fiber size and increase in connective tissue. Staining with periodic acid Schiff (PAS), NADH tetrazolium reductase, or for succinic dehydrogenase may show increased subsarcolemmal activity. Electron microscopy reveals an increase in number and size of mitochondria (Figure 51.9), some with paracrystalline inclusion bodies [13, 37].
Peripheral Blood and Bone Marrow
Published in Harold R. Schumacher, William A. Rock, Sanford A. Stass, Handbook of Hematologic Pathology, 2019
Fermina Maria Mazzella, Gerardo Perrotta
Trichrome stain is used to determine the amount of type I collagen, that is, fibrosis, that is present. Frank collagen fibrosis is absent in normal bone marrow, and uncommon in hematopoietic disorders. It does, however, occur frequently in the presence of metastatic tumor and certain acute leukemias (FAB-M7).
Enterocytozoon
Published in Dongyou Liu, Handbook of Foodborne Diseases, 2018
The two most commonly used staining techniques include the following: (1) modified trichrome stain—developed in 1992 by Weber,83 which makes use of chromotrope 2R. It is based on Wheatley's modification of the Gomori's stain with two important modifications, using chromotrope 2R at 10× concentration and increasing the staining period. These steps not only expedited the staining process but also provided a good contrast against the background. The Weber's trichrome stain stains the spore of E. bieneusi reddish-pink in color, which appear as rosy oval bodies with a nearly colorless posterior vacuole. The polar filament may take up stronger stain giving a darker contrast at the equator of the spore. The background takes the color of the counterstain used, malachite green, Fast green or aniline blue may be used for the same.2,79,82
Attenuation of streptozotocin induced high fat diet exacerbated dyslipidemia mediated hepatic and aortic injuries in male pigs by camel milk
Published in Egyptian Journal of Basic and Applied Sciences, 2023
Hadiza Bello Rilwan, Sunday Samuel Adebisi, James Abrak Timbuak, Sunday Blessing Oladele, Aliyu Muhammad, Wusa Makena, Adamu Abubakar Sadeeq
Masson’s trichrome staining was done with a ready-to-use kit (Trichrome Stain Kit). In a nutshell, the tissue slices were sliced into 5 m thick pieces and put on typical microscopy slides. After deparaffinisation and rehydration, the slides were submerged in Bouin’s solution for 15 minutes at 56°C. The slides were then rinsed with tap water for 5 minutes. The slices were then stained with Weigert’s hematoxylin for 5 minutes before being washed in tap water for 5 minutes and rinsed in distilled water. The slides were then stained for 5 minutes with Biebrich scarlet-acid fuchsin, washed in distilled water, incubated in phosphotungstic-phosphomolybdic acid for 5 minutes, colored with aniline blue for 5 minutes, and fixed in 1% acetic acid for 2 minutes. Finally, the slides were rinsed in distilled water, dehydrated, and mounted. Finally, the slides were dehydrated and mounted after being rinsed in distilled water [31]. The tissues were then viewed under a light microscope, and photomicrographs were taken with a digital microscope camera (AmScope MD 900) at a magnification of ×250.
Histological and biochemical evaluation of the effects of silver nanoparticles (AgNps) versus titanium dioxide nanoparticles (TiO2NPs) on rat parotid gland
Published in Ultrastructural Pathology, 2023
Sara M. Abdel Aal, Maha Z. Mohammed, Abeer A. Abdelrahman, Walaa Samy, Ghadeer M. M. Abdelaal, Raghda H. Deraz, Shaimaa A. Abdelrahman
Thereafter, all sections were incubated with the streptavidin – biotin peroxidase complex. Reactions were visualized with 3,30-diaminobenzidine-tetrahydrochloride (DAB – Sigma-Aldrich Chemical Co., St. Louis, USA) as a chromogen. The sections were counterstained with Mayer’s hematoxylin, dehydrated and mounted. For negative control, the primary antibody was replaced with PBS. Sections were examined and photographed with a microscope (Leica, Germany). Positive reactions for Caspase-3 and CD68 proteins appeared brown-yellow particles in the cytoplasm. Sections stained with H&E, Mallory trichrome stain, and immunohistochemistry were examined and photographed by the light microscope at the Medical Histology and Cell Biology department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
Histopathological, physiological and biochemical assessment of resveratrol nanocapsules efficacy in bleomycin-induced acute and chronic lung injury in rats
Published in Drug Delivery, 2022
Neama M. Albanawany, Doaa M. Samy, Noha Zahran, Riham M. El-Moslemany, Shefaa Mf. Elsawy, Maha W. Abou Nazel
The first part of lung tissue was cut in pieces of 0.5 mm thickness, fixed in 10% formol saline and processed to get 5 μm thick serial paraffin sections. These sections were stained with hematoxylin-eosin (H&E) or modified Masson’s trichrome stains. Histological sections were examined using light microscope (Olympus BX41) equipped with spot digital camera (Olympus DP20). Histomorphometric analysis for inter-alveolar septal thickness (μm) and alveolar surface area (μm2) of H&E stained sections and area percentage (%) of deposited collagen in Masson’s trichrome stained sections was performed using NIH Fiji program (NIH, Bethesda, MD, USA). Immunohisto-staining