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Enterocele
Published in Linda Cardozo, Staskin David, Textbook of Female Urology and Urogynecology - Two-Volume Set, 2017
Figure 83.4 The MAllory trichrome stAin of A biopsy tAken from the tissue used in posterior vAginAl wAll repAirs. The biopsy site wAs ApproximAtely 2 cm below the ischiAl spine. This stAin is used to differentiAte fibrous tissue (green) And smooth muscle (red). note the Amount of smooth muscle, orgAnized connective tissue, And AreolAr tissue. (Courtesy of Dr. Christopher MAher.)
Histological and biochemical evaluation of the effects of silver nanoparticles (AgNps) versus titanium dioxide nanoparticles (TiO2NPs) on rat parotid gland
Published in Ultrastructural Pathology, 2023
Sara M. Abdel Aal, Maha Z. Mohammed, Abeer A. Abdelrahman, Walaa Samy, Ghadeer M. M. Abdelaal, Raghda H. Deraz, Shaimaa A. Abdelrahman
Thereafter, all sections were incubated with the streptavidin – biotin peroxidase complex. Reactions were visualized with 3,30-diaminobenzidine-tetrahydrochloride (DAB – Sigma-Aldrich Chemical Co., St. Louis, USA) as a chromogen. The sections were counterstained with Mayer’s hematoxylin, dehydrated and mounted. For negative control, the primary antibody was replaced with PBS. Sections were examined and photographed with a microscope (Leica, Germany). Positive reactions for Caspase-3 and CD68 proteins appeared brown-yellow particles in the cytoplasm. Sections stained with H&E, Mallory trichrome stain, and immunohistochemistry were examined and photographed by the light microscope at the Medical Histology and Cell Biology department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
Evening primrose oil attenuates oxidative stress, inflammation, fibrosis, apoptosis, and ultrastructural alterations induced by metanil yellow in the liver of rat: a histological, immunohistochemical, and biochemical study
Published in Ultrastructural Pathology, 2023
Amany Mohamed Shalaby, Rania H. Shalaby, Mohamed Ali Alabiad, Doaa I. Abdelrahman, Mohammed Alorini, Fatima A. Jaber, Shaimaa Mohamed Abdelfattah Hassan
Each animal’s liver specimens were fixed in 10% buffered formalin solution for 24 hours before being dehydrated in graded alcohol and embedded in paraffin. The following processes were carried out on sections of 5 μm thickness: Hematoxylin and eosin (H&E) stain to show the liver’s overall histological structure.16Mallory’s trichrome stain to show the collagen fibers.17Immunohistochemical stains using antibodies against iNOS (as an inflammatory marker), GFAP (a marker for activated hepatic stellate cells), and Bax (as an apoptotic marker).
Effect of ozone versus naringin on testicular injury in experimentally induced ulcerative colitis in adult male albino rats
Published in Ultrastructural Pathology, 2022
Abeer M. Azmy, Bassant T. Abd Elbaki, Mohammed A. Ali, Abeer A Mahmoud
According to Kiernan, samples from the descending colon and testes were processed to create paraffin blocks.35 Serial sections (5 μm thickness) were subjected to the following stains: Hematoxylin and Eosin stain for both colon and testes.Mallory trichrome stain for detection of collagen fibers in testes only.Immunohistochemical staining for p53 in testes only, a marker for apoptosis is a rabbit polyclonal antibody (catalog number ab131442, Abcam, Cambridge, UK).