Study of functional drug-eluting stent in promoting endothelialization and antiproliferation
Published in Journal of Biomaterials Science, Polymer Edition, 2020
Ruixia Hou, Leigang Wu, Yabin Zhu, Jin Wang, Zhilu Yang, Qiufen Tu, Nan Huang
Human umbilical vein ECs were obtained from the umbilical arteries of newborns, kindly provided by the Women and Children Healthcare Centre of Chengdu. ECs were cultured in M199 medium containing 10% FBS in a humidified atmosphere of 95% air and 5% CO2 at 37 °C. The culture medium was changed every 2 days and the cells were passaged through trypsinization. After culturing for 1 and 3 days, samples were washed three times using PBS, and fixed in 2.5% glutaraldehyde solution for 12 h. After dehydrating, dealcoholizing and critical point drying, the samples were sputter-coated with gold, and imaged through scanning electron microscopy (SEM, Quanta200, Philips, Amsterdam, Netherlands).