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Experimental study on the influencing factors of repairing white marble beam by MICP
Published in Renato Lancellotta, Carlo Viggiani, Alessandro Flora, Filomena de Silva, Lucia Mele, Geotechnical Engineering for the Preservation of Monuments and Historic Sites III, 2022
Jing Qiao, Jianhong He, Xichen Xu, Hongxian Guo, Xiaohui Cheng
The urease active strain of Sporosarcina pasteurii (ATCC11859) was selected to induce urease hydrolysis in this study. Sporosarcina pasteurii was cultivated in yeast extract based medium (20 g/L yeast extract, 10 g/L (NH4)2SO4, 10 μM NiCl2, pH 8.5-9.0). After 14-16 hours of incubation at 30 ℃ with a continuous shaking speed of 180 r/min, the bacterial suspension was harvested. The average urease activity and the optical density (OD600) of the harvested culture are 15 mM/min and 3 respectively.
Measuring stiffness of soils in situ
Published in Fusao Oka, Akira Murakami, Ryosuke Uzuoka, Sayuri Kimoto, Computer Methods and Recent Advances in Geomechanics, 2014
Fusao Oka, Akira Murakami, Ryosuke Uzuoka, Sayuri Kimoto
Recently, Microbial Induced Calcite Precipitation, MICP, has been studied extensively for its possibility as a novel soil improvement technique. This technique utilizes the whole bacterial cells containing urease; e.g., Sporosarcina pasteurii (van Paassen et al. 2010) to dissociate urea into the carbonate compound (Eq. 1). The carbonate compound combines with the calcium ion, supplied in the form of solution of calcium salt, to form precipitate of calcium carbonate (Eq. 2). CONH22+2H2O→2NH4++CO32-CO32-+Ca2+→CaCO3
Review on biologically based grout material to prevent soil liquefaction for ground improvement
Published in International Journal of Geotechnical Engineering, 2019
Deepika Kumari, Wei-Ning Xiang
Biogrouting reinforcement to repair the impairment of masonry structures was studied by Yang and Cheng (2013). They used urease-producing Sporosarcina pasteurii and injected along with nutrient media and cementation solution into mixture of cement and lime to induce the precipitation of calcium carbonate in this system to form a microbial mortar of significant strength that reached the maximum uniaxial compressive strength up to 55 MPa. This research enabled the use of biogrouting technique with feature of being low pressured compared to conventional grouts to prepare high-strength bacterial mortar.