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Attenuation of streptozotocin induced high fat diet exacerbated dyslipidemia mediated hepatic and aortic injuries in male pigs by camel milk
Published in Egyptian Journal of Basic and Applied Sciences, 2023
Hadiza Bello Rilwan, Sunday Samuel Adebisi, James Abrak Timbuak, Sunday Blessing Oladele, Aliyu Muhammad, Wusa Makena, Adamu Abubakar Sadeeq
The attached camera to the microscope was used to record five sections per pig and five random fields for each section (totaling 25 fields). Using Kleiner et al.’s [32] scoring system, the two important features examined in the H&E liver were steatosis and inflammation, whereas in the aorta they were tunica intima ulceration and medial cystic necrosis. The severity of the liver steatosis, aortic ulceration, and medial cystic necrosis was graded based on the percentage of the overall area affected, while inflammation was measured by counting the number of inflammatory foci per field at 100 X magnification. The hepatic reticular fiber loss and aortic collagen disrupted-positive areas in the liver and aorta sections were measured for histochemistry. As previously reported by Ruifrok and Johnston [33], color deconvolution was used to quantitatively analyze the photomicrographs using Image J software.