China
Africa
Explore chapters and articles related to this topic
Studying Biologically Templated Materials with Atomic Force Microscopy
Published in Sivashankar Krishnamoorthy, Krzysztof Iniewski, Nanomaterials, 2017
Andrew J. Lee, Christoph Walti
A promising approach is to utilize the Escherichia coli protein Recombinase A (RecA) for spatially addressing DNA architectures.54 The RecA protein is involved in DNA repair pathways through homologous recombination. It polymerizes upon single-stranded DNA in the presence of ATP and magnesium to form a nucleoprotein filament. Subsequently, this nucleoprotein complex is able to self-assemble onto a double-stranded DNA at regions of shared homology between the encapsulated single-stranded DNA and the double-stranded DNA (Figure 3.5a). Upon successful assembly, the two homologous strands exchange resulting in the substitution of the new single-stranded DNA molecule with the homologous region of the double-stranded DNA molecule, and then the RecA complex disassociates through the hydrolysis of ATP.
Multiple inhibitory effects of succinic acid on Microcystis aeruginosa: morphology, metabolomics, and gene expression
Published in Environmental Technology, 2022
Yi-dong Chen, Chu Zhao, Xiao-yu Zhu, Yuan Zhu, Ru-nan Tian
The recA gene encodes a highly conserved and multifunctional RecA protein, which plays a key role in DNA repair and initiates the SOS response to DNA damage [41]. Studies have shown that the expression of recA will be up-regulated when the environmental stress is not serious but down-regulated under severe environmental stress [42, 43]. In this study, the recA transcription decreased significantly after treatment with 60 mg L−1 SA for 3 h, and then returned to the control level at 24 h. It is possible that the Microcystis cells sensed the SA stress in a short period of time, which caused the expression to decrease. With extended exposure time, the cells became adapted to this stress, and the expression returned to the control level.