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Colonization of Iron- and Zinc-Contaminated Dumped Filtercake Waste by Microbes, Plants, and Associated Mycorrhizae
Published in M.H. Wong, J.W.C. Wong, A.J.M. Baker, Remediation and Management of Degraded Lands, 2018
T.M. Chaudhry, L. Hill, A.G. Khan, C. Kuek
The microbial populations of the contaminated (filtercake) and uncontaminated soil samples were determined by serial dilution and plating of soil suspensions on differential culture media. For total bacterial count, plate count agar (PCA) plates were used. Sabouraud agar was used to isolate and enumerate total fungal population from soil samples of the contaminated and uncontaminated sites (Harley and Prescott, 1993).
Multifunctionalization of self-assembled silver nanoparticle coated poly(ethyleneimine)/poly(diallyldimethylammonium chloride) modified silk fabric
Published in The Journal of The Textile Institute, 2022
Xianglei Wang, Ce Cui, Ang Li, Jianyu Zhai, Jing Wu, Ronghui Guo, Erhui Ren, Hongyan Xiao, Mi Zhou, Xueqing Yang, Jinwei Zhang
Gram-positive S. aureus and gram-negative E. coli) were used for the antibacterial tests. In order to prepare standard S. aureus and E. coli solution, colonies of S. aureus and E. coli were first cultivated on a culture medium, respectively, and incubated at 37 °C for 18 h. Culture mediums were prepared by adding 2.35 g plate count agar into 100 mL deionized water and 15 mL plate count agar solution was poured into each culture dish and coagulated after sterilizing in an autoclave at 121 °C for 15 min. Colonies of S. aureus and E. coli were then taken from culture mediums and added into a 100 ml of nutrient broth (NB) solution autoclaved at 121 °C for 15 min, respectively. The suspension was incubated 18 h in a shaker incubator at 37 °C and 160 rpm.
Monitoring the effect of environmental conditions on safety of fresh produce sold in Qatar’s wholesale market
Published in International Journal of Environmental Health Research, 2022
I. Elnemr, M. Mushtaha, Sathyavathi Sundararaju, Mohammad Rubayet Hasan, Kin-Ming Tsui, I. Goktepe
About 5 ml of each homogenized mixture was added to the same volume of sterile Brain Heart Infusion broth and incubated at 37°C with continuous shaking at 150 rpm for about 3–6 hrs to enrich the microorganisms as described by El-Nemr et al. (2019a). Exactly 0.1 ml of each diluted sample was spread in duplicate on the following selective media: i) Plate-Count Agar (PCA) used for total aerobic bacterial (TAB) counts, ii) Baird-Parker Agar (BPA) supplemented with egg yolk for Staphylococcus spp., iii) Eosin Methylene Blue Agar (EMBA) for total coliforms, iv) Listeria Selective Agar (LSA) for Listeria spp., v) MacConkey Agar (MCA) to grow E. coli and other gram negative enteric bacilli, vi) Xylose Lactose Tergitol 4 agar (XLT4) for Salmonella spp., vii) Potato Dextrose Agar (PDA) for total fungi, and viii) Rose Bengal Chloramphenicol Agar (RBCA) for enumeration of yeasts and molds. All media used in this study were purchased from Oxoid Ltd. Hampshire, UK.