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Host Response to Biomaterials
Published in Claudio Migliaresi, Antonella Motta, Scaffolds for Tissue Engineering, 2014
Sangeetha Srinivasan, Julia E. Babensee
An interesting, biomaterial chemistry-based angiogenesis approach has been described wherein porous bulk polymerised SCs prepared from n-butyl methacrylic acid-methyl methacrylate (MMA) co-polymer demonstrated an ability to promote angiogenesis within a 30-day period when implanted in rats.169 This approach is interesting since the angigogenic observation is dependent solely on a unique material-chemistry (methacrylic acid [MAA] containing) without the additional of any exogenous factors such as angiogenic growth factors. Beads made of the same material were used to treat skin grafts in rodents with an observed benefit on graft engraftment.170 This was associated with a significantly increased microvessel density and thickness of panniculus carnosus muscle 11 days post implantation. In a similar study, poly(MAA-co-MMA) (45 mol% MAA) beads enhanced wound closure and vascularization in diabetic mice within a 21-day period.171 Another study compared the effect of surface charge properties on angiogenesis. Surface-modified polymer fibers were plasma coated with either negatively charged MAA, positively charged N,N-dimethyl-aminoethyl methacrylate, or neutral hexafluoropropylene.172-174 In vivo implantation showed that MAA-coated constructs promoted angiogenesis better than the other groups. This suggests a possible dependence of angiogenic behavior on the negative charge carried by MAA. Furthermore, other material properties such as polymer fiber diameter and fiber spacing within SC mesh were shown to directly impact the development of neovascular structures.
Epidermal stimulating factors-gelatin/polycaprolactone coaxial electrospun nanofiber: ideal nanoscale material for dermal substitute
Published in Journal of Biomaterials Science, Polymer Edition, 2021
Li Yan, Haoyu Wang, Hui Xu, Rui Zheng, Zhengyu Shen
The recovery of healthy epidermal and dermal structure in our treated tissues was assessed by measuring the epidermal thickness using haematoxylin and eosin (H&E) staining and Masson's trichrome staining tissue sections. The thickness of epidermal was calculated between two lines (Supplementary material Figure S3A). Lower line was drawn at the interface of the dermis and the stratum basal, and the upper line was positioned above the stratum granulosum, disregarding the stratum corneum as it flaked off during staining. The thickness of dermal was calculated between the stratum basal and the lower margin of the coherently arrayed collagens (Supplementary material Figure S3B) which can be stained blue by Masson’s trichrome staining. The thickness was measured manually using ImageJ image analysis software. The mean thickness was calculated by the area of epidermal or dermal versus the wound length. The wound edges were defined by determining the position of the underlying panniculus carnosus muscle tissue.
Wound healing performance of PVA/PCL based electrospun nanofiber incorporated green synthetized CuNPs and Quercus infectoria extracts
Published in Journal of Biomaterials Science, Polymer Edition, 2023
Shohreh Fahimirad, Parastu Satei, Ali Ganji, Hamid Abtahi
All of the in vivo trials were authenticated agreeably by the ethical committee of Arak University of Medical Sciences. 60 adult male mature males from Westar mice were with an average body weight of 250 g were utilized for this assay. The rats were randomly allocated to 12 trial classified groups including of six animals in each group: PCL, PCL/PVA, PCL/PVA/QLG extract, PCL/PVA/CuNPs, PCL/PVA/QLG/CuNPs (with best determined bioactive concentrations based on antibacterial and antioxidant results) and control (with two-layer sterile gauze as standard common used wound dressings) under two circumstance of wounds infected and uninfected with MRSA to verify the wound healing performance of the construct nanofibers. Each rat was kept in a cage and fed separately. At first, the mice were anesthetized with 50 mg/kg ketamine and 5 mg/kg xylazine. Afterwards, the shaven area of the panniculus carnosus muscle was selected to create a 2 × 2 cm2 thickness excisional wound. The prepared MRSA cell suspension (1.5 × 108 CFU, 10 μL) was identically inoculated on the wounds. Treatment of the infected wounds was initiated 1 h after wound colonization. 2 × 2 cm2 samples were taken from nanostructured dressings, sterilized by UV and located onto the wounds surfaces of all rats except those in the control group [64]. Wound healing processes including surface exudation was assessed at days 5th, 10th and 15th. The percentage of wound healed postsurgical was calculated as follows: where A0 and Ascar are original wound and scared areas, respectively [9, 61, 65].