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Published in Valerio Voliani, Nanomaterials and Neoplasms, 2021
Nardine S. Abadeer, Catherine J. Murphy
Others have reported RF hyperthermia in vivo. Curley and coworkers [63] injected nude balb/c mice with Panc-1 or Capan-1 human pancreatic carcinoma cells to form subcutaneous tumors. 10 nm gold nanospheres were functionalized with anti-EGFR or PAM4 antibodies to target EGFR or MUC-1 receptors, respectively, on pancreatic cells. No thermal injuries were observed in mice due to RF exposure (13.56 MHz, 600 W, 10 min) because any effects of excess electric current were prevented by attaching conductive tape to the extremities of the mice. Treatment with RF and nanoparticles resulted in a bulk Panc-1 tumor temperature increase of 3°C above body temperature. After 6 weeks, tumor growth was significantly impeded with the dual treatment compared to a significant volume increase with untreated mice or nanoparticles/RF exposure alone. Mass spectrometry analysis of organs revealed that there was accumulation of the nanoparticles in the tumor (25.1 ± 6.4 mg) but also the spleen (257.6 ± 7.6 mg), liver (302.7 ± 82.5 mg), kidneys (4.1 ± 0.1 mg), and lungs (4.0 ± 0.3 mg) of mice. Surprisingly, no tissue damage was reported in those nontumor regions, even with the substantial accumulation of nanoparticles in the liver and spleen. In the Capan-1 tumors, PAM4 gold nanoparticles and RF exposure seemed to slow growth in the first few weeks but did not significantly reduce tumor size after 7 weeks. Since PAM4 is more pancreas cancer-specific than anti-EGFR, the reduced effectiveness of PAM4 gold nanoparticles in RF cancer therapy was unexpected. This suggested that the chosen nanoparticle ligand was influential in successful RF therapy [63].
Plasmonic photothermal effect on cytotoxicity of biogenic nanostructure synthesized through Litchi chinensis Sonn.
Published in Inorganic and Nano-Metal Chemistry, 2021
The pancreatic cancer cell lines (PANC-1) were used to determine the apoptosis associated protein levels. The expression of PARP protein is upregulated indicated the apoptosis in the range as 5FU AgSe NPs > AgSe > Se, > Ag nanoparticles. In this study, B-actin gene acts as internal standard and it didn’t display any changes within the expression level (Figure 7b). The observed results clearly showed that the synthesized biogenic nanoparticles facilitated apoptosis of cancer cells by increasing the protein expressions associated with apoptosis.