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Rehabilitation Computing in Electronic Computing
Published in Parveen Berwal, Jagjit Singh Dhatterwal, Kuldeep Singh Kaswan, Shashi Kant, Computer Applications in Engineering and Management, 2022
Parveen Berwal, Jagjit Singh Dhatterwal, Kuldeep Singh Kaswan, Shashi Kant
The effect of TGF- β3 and its carries on cell attachment was investigated using trypsinization. Dilutions are deposited into the three rows of a 12 well plate to determine the optimal dilution for plating cells. TGF-“3 was given enough time to affect cell cultures by growing them for at least 2 days before the attached test. The 1 in 3 titration was selected for this assay because it was confluent by day 3. A solution of HCl (4 mM), BSA (1 mg/ml), and purified water was prepared to reconstruct the vile containing TGF-“3. To investigate the effect of TGF-“3, HCl, HCl/BSA, and bone cell only as a guide on cell removal, trypsin was applied to four classes of cultivated bone cells with four separate solutions, including TGF-“3, HCl, HCl/BSA, and bones cell only as a control. TGF-“3 is carried by HCl and HCl/BSA solutions, so they were used. This procedure was carried out again for groups A, B, C, and D. The rate at which cells detach from the surface is critical, and it was possible to identify which group (A, B, C, or D) disconnected faster. To investigate the effect of TGF-“3, HCl, HCl/BSA, and bone cell only as a guide on cell attachment, trypsin was applied to three classes of cultured bone cells with four separate solutions, namely, TGF-“3, HCl, HCl/BSA, and bones cell only as a guide.
Cell Biology for Bioprocessing
Published in Wei-Shou Hu, Cell Culture Bioprocess Engineering, 2020
There are many different types of cells that constitute the tissues and form the various organs in a mammal. They all arise from the same fertilized egg and then differentiate into cells with vastly different properties, using the same set of genetic information but arriving at very different destinies. Over a century ago, scientists began to isolate cells from explanted tissues. In Chapter 1 we briefly discussed the enabling advances that made cell culture a research tool and production host, including the development of media and its membrane sterilization and cell trypsinization and passaging. Most cells that outgrew from tissues could only be cultured for a very small number of generations, but eventually some cells were successfully established that could be passaged over many generations. After cryopreservation methods were established, cells could be “banked” for future use, allowing for the generation of the reproducible results that are essential to research and manufacturing.
Microcarrier Culture Systems
Published in Anthony S. Lubiniecki, Large-Scale Mammalian Cell Culture Technology, 2018
A wide range of cells have been cultivated on various MCs (37, 40), e.g., anchorage-dependent and independent cells; cells of invertebrate, fish, bird, and mammalian origin; transformed and normal cells; fibroblastic or epithelial cells; and, lately, recombinant cell lines. A list of cells propagated on MCs was recently published by Pharmacia (92). A detailed description of a procedure for propagating cells on MCs at the lab oratory scale is described by Lindskog et al. (93). The most common method for enumerating cells in MC culture is nuclei count (36, 94). In this method a solution of 0.1% crystal violet and 0.1 M citric acid is added to the cell-covered MCs. After incubation at 37°C, the nuclei are released from the cells by vigorous mixing. The crystal violet stains the nuclei, which are counted in a hemacytometer. A variation of the nuclei count method is to lyse the cells in a 0.1 M citric acid solution and 1% Triton X-100. With this method, there is much less background debris and the nuclei can be counted in an automatic particle counter (67). Other researchers have trypsinized cells from the MCs surface and then counted them by the trypan blue exclusion methin in a hemacytometer. There is not always a good correlation between these two methods. The correlation depends on the viability of the culture and the efficiency of cell trypsinization (95).
Peptide-enabled receptor-binding-quantum dots for enhanced detection and migration inhibition of cancer cells
Published in Journal of Biomaterials Science, Polymer Edition, 2020
Ruijuan Zu, Xiaocui Fang, Yuchen Lin, Shilin Xu, Jie Meng, Haiyan Xu, Yanlian Yang, Chen Wang
The culture medium was replaced once every 2 or 3 days. Cells were cultured to about 85–90% confluence before harvest. For the harvest, cells were washed gently with PBS buffer followed by trypsinization with 0.25% (w/v) trypsin-EDTA for 3–5 min at 37 °C to detach the cells from the flask. Then, the trypsin-EDTA containing cells were neutralized by adding 2 times of fresh supplemented culture medium, followed by centrifugation at 1000 rpm for 3 min. Finally, the supernatant was discarded, and the cells were re-suspended in culture medium for the following experiments.