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Bioprinting of liver
Published in Ali Khademhosseini, Gulden Camci-Unal, 3D Bioprinting in Regenerative Engineering, 2018
Dong-Woo Cho, Hyungseok Lee, Wonil Han, Yeong-Jin Choi
Matrigel® is a commercially available hydrogel derived from murine sarcoma cells with thermal gelation properties equal to those of collagen [91]. Because of its origin, Matrigel provides cells with a more suitable microenvironment than the aforementioned materials. Many reports describe culture methods that involve Matrigel to maintain the phenotype [92] or improve functionality [93] of primary hepatocytes. Moreover, Matrigel has also been used as a biomaterial to fabricate HepG2-laden constructs [94]. Although Matrigel provides an outstanding microenvironment, it cannot fully support tissue-specific growth factors or cytokines. Furthermore, the heterogeneous qualities of Matrigel can result in variable cellular responses, and the tumor origin of Matrigel inhibits clinical applications in humans because of its tumorigenic potential [95]. Nevertheless, Matrigel is considered as one of the most effective biomaterials for liver bioprinting.
Methods and Protocols for In Vitro Animal Nanotoxicity Evaluation: A Detailed Review
Published in Vineet Kumar, Nandita Dasgupta, Shivendu Ranjan, Nanotoxicology, 2018
Venkatraman Manickam, Leema George, Amiti Tanny, Rajeeva Lochana, Ranjith Kumar Velusamy, M. Mathan Kumar, Bhavapriya Rajendran, Ramasamy Tamizhselvi
Now it is even possible to accurately bio-print human embryonic stem cells to produce spheroids of uniform size and still maintain the pluripotency of printed cells. These pluripotent stem cells can provide an unlimited source of scarce or difficult to isolate cells during drug screening and toxicity testing procedures (Faulkner-Jones et al. 2013). Due to their significant water content, hydrogels, and an extra cellular matrix (ECM, made of collagen, laminin, fibrin, hyaluronic acid, and chitosan or a Matrigel® matrix) possess biophysical characteristics very similar to natural tissue, and serve as highly effective matrices for 3D cell culture through structural support. Among the natural hydrogels, Matrigel® matrix is widely applied in 3D cell cultures especially for recreating tumor models. Likewise, solid scaffolds for 3D cell culture are fabricated with a broad range of materials including metals, ceramics, glass, and polymers using a multitude of microfabrication and microfluidics technologies (van der Worp et al. 2010; Sanyal 2014).
Biodegradable Hydrogels: Tailoring Properties and Function through Chemistry and Structure
Published in Joyce Y. Wong, Joseph D. Bronzino, Biomaterials, 2007
Andrew T. Metters, Chien-Chi Lin
Modified collagen gels are still favored for many tissue-engineering applications. Composite scaffolds such as collagen-alginate [Bohl et al., 1998] or collagen-hyaluronan [Segura, Anderson et al., 2005;Segura, Chung et al., 2005] have been fabricated and used for several tissue engineering and DNA delivery applications. Matrigel, a type IV collagen-based and a commercially available hydrogel, mimics the ECM environment and is commonly used in in vivo or in vitro studies including cell growth and differentiation [Taub et al., 1990], angiogenesis, and tumor augmentation [Benelli and Albini, 1999; Auerbach et al., 2003]. Collagen hydrogels have also been used to immobilize human neuroblastoma cells for cell-based biosensing [Mao and Kisaalita, 2004].
Culture of pyramidal neural precursors, neural stem cells, and fibroblasts on various biomaterials
Published in Journal of Biomaterials Science, Polymer Edition, 2018
Mo Li, Ying Wang, Jidi Zhang, Zheng Cao, Shuo Wang, Wei Zheng, Qian Li, Tianqi Zheng, Xiumei Wang, Qunyuan Xu, Zhiguo Chen
Matrigel was purchased from BD (Cat.354277, BD). Matrigel was a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in such extracellular matrix (ECM) proteins as laminin (a major component), collagen IV, heparin sulfate proteoglycans, entactin/nidogen, and a number of growth factors.