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Microencapsulation of Living Cells and Tissues — Theory and Practice
Published in Franklin Lim, Biomedical Applications of Microencapsulation, 2019
HL-60 cells, a myeloid cell line derived and maintained originally by Collins and co- workers,19,20 from a patient with acute promyelocytic leukemia, have been observed to proliferate and continue to survive for over 15 months after microencapsulation. These promyelocytic leukemia cells can continue to grow inside microcapsules until they are all tightly packed before eventually breaking loose by bursting the capsular membrane. The high degree of cell-packing can be seen in Figure 12.
Algae as Food and Nutraceuticals
Published in Sanjeet Mehariya, Shashi Kant Bhatia, Obulisamy Parthiba Karthikeyan, Algal Biorefineries and the Circular Bioeconomy, 2022
Chetan Aware, Virdhaval Nalavade, Rahul Jadhav, Shashi Kant Bhatia, Yung-Hun Yang, Jyoti Jadhav, Ranjit Gurav
Fucoxanthin is a xanthophyll that formed by an unusual allenic bond and 5,6-monoepoxide within the structure. Amongst the most common carotenoids present in plants and algae is fucoxanthin (Matsuno, 2001). The composition of seaweed differs depending on the environmental conditions and development process. Besides destruction in the drying method and preservation at the usual temperature, it is very stable over the stress of organic components. Fucoxanthin is prone to oxidation in its natural form (Haugan and Liaaen-Jensen, 1994). Fucus serratus has a gross carotenoid level of about 0.08% of the dried harvested samples, with fucoxanthin accounting lasting for approximately 70% (Haugan and Liaaen-Jensen, 1994). Fucoxanthin abundance in brown algal species varies from 170–750mg/kg dry mass, with the greatest concentration in F. serratus L. (Tsukui et al., 2007). However, values of 3700mg/kg have been recorded in Sargassum horneri (Turner) C.Agardh (Tsukui et al., 2007). When provided in drinkable water, fucoxanthin from Undaria predominantly decreases the proliferation of prostate cancer in humans, as well as the proportion of malignant cells in animal cancer models and the total number of tumors per animal (Miyashita and Hosokawa, 2008). Recent investigations have shown anticancer influence, comprising prevention of human leukemia cell line multiplication (HL-60), and have led to apoptotic cell death (Miyashita and Hosokawa, 2008). The decrease of white adipose tissue in rodents and obese diabetic mice model was illustrated by Maeda et al. (2005), once fed with fucoxanthin from brown algae. Fucoxanthin-rich diets have reduced total cholesterol in mice and increased the expression of a thermoregulatory protein in adipose tissues. Extracted fucoxanthin prevented cellular lipid uptake in 3T3-L1 adipocytes. This indicates that fucoxanthin is a useful organic component for preventing obesity (Miyashita and Hosokawa, 2008). Antioxidant function, anti-inflammatory potential, neuroprotective activities, antiangiogenic properties, and skin defensive influence are among the certain biological functions attributed to fucoxanthin (Miyashita et al., 2012).
Antimicrobial and antileukemic effects: in vitro activity of Calyptranthes grandifolia aqueous leaf extract
Published in Journal of Toxicology and Environmental Health, Part A, 2020
Fernanda Majolo, Shanna Bitencourt, Bruna Wissmann Monteiro, Gabriela Viegas Haute, Celso Alves, Joana Silva, Susete Pinteus, Roberto Christ Vianna Santos, Heron Fernandes Vieira Torquato, Edgar Julian Paredes-Gamero, Jarbas Rodrigues Oliveira, Claucia Fernanda Volken De Souza, Rui Felipe Pinto Pedrosa, Stefan Laufer, Márcia Inês Goettert
RAW 264.7 murine macrophage, epithelial cells from CHO-K1 Chinese hamster ovary and human colon adenocarcinoma Caco-2 cell lines were obtained from the Banco de Células do Rio de Janeiro (BCRJ), and leukemia cell lines (HL60, K562, and Kasumi-1) were obtained from the American Type Culture Collection (ATCC, USA). RAW 264.7 and Caco-2 cells were cultured in DMEM medium and CHO-K1 cells in DMEM + Nutrient Mixture F-10 Ham medium (Ham’s F-10) (Sigma-Aldrich). All cell lines were supplemented with 10% fetal bovine serum (FBS) and 1% antibiotics (100 U/ml penicillin G and 100 µg/ml streptomycin). HL60, K562, and Kasumi-1 cells were cultured in RPMI-1640 medium supplemented with 10% FBS and 1% antibiotics. Cells were incubated at 37°C in a humidified atmosphere containing 5% CO2. Preliminary cell viability was determined by the exclusion method with trypan blue.