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Advanced Systolic Design
Published in Keshab K. Parhi, Takao Nishitani, Digital Signal Processing for Multimedia Systems, 2018
Dominique Lavenier, Patrice Patrice, Sanjay Rajopadhye
Example of systolic arrays designed using FPGA boards are: systolic filter for fast DNA bank scanning [49],2D convolution [50],text searching [51],numerical algorithms [52], division and other arithmetic operations [53].
Developing mitochondrial DNA field-compatible tests
Published in Critical Reviews in Environmental Science and Technology, 2022
Bidhan C. Dhar, Christina E. Roche, Jay F. Levine
Environmental samples pose an additional challenge. Mitochondrial DNA concentrations decline rapidly after entering surface waters. Microbial DNA and vertebrate mtDNA in surface waters is nonuniformly distributed and subject to degradation by environmental processes (Dick et al., 2010; He et al., 2016). The eDNA fragment size varies from species to species and with environmental conditions (Moushomi et al., 2019), consequently, the microscopic pore size of filters must be optimized to effectively capture eDNA (Hinlo et al., 2017). Ambient light, temperature fluctuations, extremes in acidity, and microfaunal biologic processing degrade mtDNA and diminish the quantity of mtDNA that can be detected in environmental samples. To improve mtDNA stability, a filter paper DNA bank card (DBC®) (KBC, IR Iran) has proven useful for storage of environmental DNA (eDNA) (Karimian et al., 2011). Another study focused on detecting Campylobacter spp. pathogenic to houseflies (Musca domestica Linnaeus) demonstrated that mtDNA can be collected from lysed cells, stored and preserved on Whatman® FTA classic cards (Clifton, NJ, USA) filter paper prior to PCR-based detection of mtDNA targets (Owens & Szalanski, 2005). Preservation of nucleic acids on filter paper can facilitate storage under ambient conditions and simplifies the sharing of nucleic acids with other researchers.