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Production of Biomass-Based Butanol
Published in Jitendra Kumar Saini, Surender Singh, Lata Nain, Sustainable Microbial Technologies for Valorization of Agro-Industrial Wastes, 2023
Cai et al. (2016) reported the dilute alkaline NaOH treatment of cob, leaf, and stem hydrolysate, flower, and husk of corn produced 9.4 g/L, 7.6 g/L, 7.5 g/L, and 7 g/L butanol respectively by C. acetobutylicum ABE 1301. Valles et al. (2021) optimized butanol production from rice straw by NaOH pretreatment (0.75% w/v) at 134°C for twenty minutes by Clostridium beijerinckii. Butanol production by C. acetobutylicum NRRL B-591 was also improved with organosolv pretreatment of rice straw by aqueous ethanol 75% (v/v) added with 1% w/w sulfuric acid at 180oC for thirty minutes (Amiri et al. 2014). The combination of dilute acid and oxidative ammonolysis increased enzymatic hydrolysis in sugarcane bagasse by C. acetobutylicum CH02. Butanol production was improved to 7.9 g/L from acid hydrolysates of barley straw by C. acetobutylicum DSM 1731 with polyethylene glycol PEG 4000 surfactant-assisted xylanase and cellulase treatment (Yang et al. 2017). Higher butanol was produced by Clostridium beijerinckii DSM 6422 from hydrolysates of wheat straw by steam explosion and ozone treatment (Plaza et al. 2017). Clostridium saccharobutylicum DSM 13864 produced butanol efficiently from pretreatment of corn stover with ten times recycled ionic liquid [Bmim][Cl] (Ding et al. 2015). Butanol production by microwave-assisted alkali pretreatment and enzymatic hydrolysis was studied by Valles et al. (2020).
Bioconversion of sago effluent and oil cakes for bio-butanol production using environmental isolates
Published in Biofuels, 2021
The microorganisms were isolated from solid pith sago waste collected from sago factories’ waste disposal sites [22]. The screening of facultative anaerobic carbohydrate fermenters was performed using OF medium whivh gave a positive result by showing a change in color (green to yellow). The strain that gave a positive result was subjected to butanol screening. The strain turned bluish green when treated with Jones reagent. The isolate also gave a positive result showing a zone indicating the hydrolysis of starch in the medium. The isolated strain was subjected to 16S rRNA analysis which indicated 98% identity with Enterococcus hirae (GenBank accession number KX261206). There are previous reports of isolation of Enterococcus spp. from soil and waste [23]. Previous studies have reported the isolation of biofuel producers such as Clostridium saccharobutylicum, which has the ability to produce butanol [24]. Ravinder et al. reported in their study the isolation of solvent-producing bacteria from sago industry waste sites [25]. ABE-producing Clostridia have been isolated from various sources, identified since 1980s. [26].