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Potentials of Polyhydroxyalkanoates as Materials for Constructing Cell Scaffolds in Tissue Engineering
Published in Tatiana G. Volova, Yuri S. Vinnik, Ekaterina I. Shishatskaya, Nadejda M. Markelova, Gennady E. Zaikov, Natural-Based Polymers for Biomedical Applications, 2017
Tatiana G. Volova, Yuri S. Vinnik, Ekaterina I. Shishatskaya, Nadejda M. Markelova, Gennady E. Zaikov
There are several approaches to the application of cell technologies to tissue repair. One of them is to introduce cell suspension of the required phenotype of definite concentration grown in vitro into the damaged tissues or into blood. Another, more technologically complex, approach is to grow cells in vitro, on a scaffold, and then implant the bioengineered construct or the new tissue to the recipient organism. Successful implementation of this approach depends on the properties of cell scaffolds (Wang et al., 2003; Hench and Jones, 2007).
3D collagen porous scaffold carrying PLGA-PTX/SDF-1α recruits and promotes neural stem cell differentiation for spinal cord injury repair
Published in Journal of Biomaterials Science, Polymer Edition, 2023
Zhixiang Li, Panpan Xu, Lijun Shang, Bingxu Ma, Huihui Zhang, Liangmin Fu, Yuanyuan Ou, Yingji Mao
NSCs were isolated from the fetal brains of embryonic 24-h Sprague-Dawley (SD) rats as previously described [37] and cultured in serum-free DMEM/F12 growth medium (Gibco) containing 2% B27 (Gibco), 20 ng/mL bFGF (Peprotech, Suzhou, China), 20 ng/mL EGF (Peprotech), and 1% penicillin and streptomycin (Beyotime Biotechnology). Briefly, bilateral cerebral hemispheres were dissected and dissociated. After stripping the meninges, all tissues were cut into small pieces and filtered using a 70-μm cell strainer (Biosharp, Beijing, China), followed by centrifugation at 800 rpm for 10 min. Thereafter, the supernatant was discarded, and the cell pellet was dissolved in a serum-free growth medium to obtain a cell suspension. The cell suspension was then transferred to a cell incubator in a 5% CO2, 37 °C environment.
Effect of phosphodiester composition in polyphosphoesters on the inhibition of osteoclastic differentiation of murine bone marrow mononuclear cells
Published in Journal of Biomaterials Science, Polymer Edition, 2023
Sota Fukaura, Yasuhiko Iwasaki
The animal experiment protocol was approved by the animal experimentation committee of Kansai University (Permit Number: 2208). Mouse bone marrow (BM) cells were isolated from the long bones (humerus, femur, and tibia) of two donor mice by modifying a previously described method [29]. The BM cell suspension was prepared with alpha-MEM containing 10% fetal calf serum (FCS) and 100 U/mL penicillin–streptomycin. After removing bigger particles by sedimentation for a few minutes, the BM cell suspension was transferred to a new tube and centrifuged. The BM cells were suspended in a fresh medium, and the suspension was seeded into a cell culture dish. After 2 h, nonadherent cells were collected, and BMNCs were separated using Ficoll 1.084. M-CSF and PGE2 were added to the suspension of BMNCs (1.67 × 105 cells/mL, 10 mL) to achieve a final concentration of 10 ng/mL and 10−7 M, respectively. The cell suspension (600 µL) was added to a 48-well cell culture plate and incubated at 37 °C, 5% CO2 for 3 days. After 300 µL of medium was removed from each well, 240 µL of fresh medium containing M-CSF, PGE2, and RANKL, and 60 µL polymer/PBS were added to the well. The final concentrations of M-CSF, PGE2, and RANKL were adjusted to 10 ng/mL, 0.1 µM, and 10 ng/mL, respectively. In contrast, the final concentration of polymers varied from 0 to 0.5 mg/mL. After 1-day cultivation, the viability of cells in contact with polymers was investigated using a WST-8 Cell Counting Kit (Dojindo, Kumamoto, Japan).
Anaerobic biodegradation of pyrene by Klebsiella sp. LZ6 and its proposed metabolic pathway
Published in Environmental Technology, 2020
Xiang Li, Xueying Zhang, Lian Li, Chaoba Lin, Weiliang Dong, Weiran Shen, Xiaoyu Yong, Honghua Jia, Xiayuan Wu, Jun Zhou
An aliquot of cell suspension as described in part 2.4 was added to the respective growth medium. In order to study the effects of different inoculation proportions on pyrene degradation at 30°C, the strain was inoculated into 10 mL of IM (pH = 7.2) contained 50 mg/L pyrene at the volume proportions of 1%, 5%, 10%, and 20%. The strain count in the inoculation seed liquid was 6.5 × 108 cells/mL. The pH values of the medium were adjusted to 4.8, 6.0, 7.2, 8.4, and 9.6 in order to investigate the pH on the anaerobic degradation of pyrene. The inoculation proportion was 10%, and other conditions remained unchanged. To study the effect of temperature on pyrene degradation, these cultures were incubated at 22, 30, 38, and 50°C. At last, the concentration of pyrene was adjusted to 50, 100, 150, and 200 mg/L, and other conditions remained unchanged in order to investigate the initial concentration of pyrene on the anaerobic degradation of pyrene.