China
Africa
Explore chapters and articles related to this topic
Detection of Food, Agricultural and Aquatic Contaminants
Published in Richard O’Kennedy, Caroline Murphy, Immunoassays, 2017
Marie Le Berre, Caroline Viguier, Caroline Murphy, Niamh Gilmartinb
The early detection of diseases such as bovine tuberculosis and foot-and-mouth disease is critical for the meat industry. Cui et al. [51] developed a rapid and sensitive impedance-based immunosensing method for the detection of antibodies produced due to tuberculosis infection with a limit of detection of 10 ng mL−1. However, distinguishing infected animals from vaccinated animals is a challenge. Whelan et al. [52] reported the performance of a novel multiplex serological test with sera collected from skin-tested animals with bovine tuberculosis, TB-free animals, and cattle vaccinated with Mycobacterium bovis BCG. The results showed the test to have a sensitivity range of 77.0–86.5% and thus this assay is suitable for distinguishing vaccinated from infected animals. A similar approach was shown by Gao and colleagues [53] to differentiate infected from vaccinated animals with foot-and-mouth disease virus. An indirect ELISA was developed based on the recombinant 8BF protein and the sensitivity of the assay was 96.8% and the specificities for naive and vaccinated cattle were 99.8% and 99.0%, respectively.
Molecular characterization and antimicrobial resistance profiles of Mycobacterium tuberculosis complex in environmental substrates from three dairy farms in Eastern Cape, South Africa
Published in International Journal of Environmental Health Research, 2021
Athini Ntloko, Martins Ajibade Adefisoye, Ezekiel Green
Bovine tuberculosis is a zoonosis infectious disease caused by M. bovis and M. caprae, which are members of the Mycobacterium tuberculosis complex (MTBC), their natural hosts being domestic and wild mammals (Brites et al. 2018). Contamination of the environment with MTBC has long been considered a key factor for the persistence of bTB in a multiple-host-pathogen system. This, coupled with the emergence and spread of MDR-TB strains and the burden of HIV/AIDS, as well as the challenges in TB diagnosis due to methodological issues, has made the management of TB problematic, particularly in developing countries (Cox et al. 2011).