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Development and Optimization of Preservation Solutions
Published in John J. Lemasters, Constance Oliver, Cell Biology of Trauma, 2020
Our group modified the gluconate perfusion fluid developed for the kidney and used this to preserve, first, the pancreas,21 then, the liver22 and kidney.23 The perfusate was modified by substitution of lactobionate for gluconate (Table 1). As lactobionate has a molecular mass (358 Da) about twice that of gluconate, we felt it would be less likely to enter the cell during cold static storage. Raffinose, a trisaccharide, was also added to increase the osmolality to 320 mOsm/l. Furthermore, the gluconate kidney perfusate was an extracellular-type solution (high relative to K), and the new cold-storage solution was made with a high K content (120 mM). The composition of this solution (University of Wisconsin Solution, UW Solution, ViaSpan) is shown in the table. It remained similar to the kidney perfusion fluid in terms of the presence of allopurinol, magnesium, adenosine, HES, and glutathione. With this solution, dog livers were successfully transplanted after two-day storage, and the kidney and pancreas after three-day storage. Furthermore, it appeared to be a reasonable choice for extended heart preservation, and successful 15-h preservation was achieved.23
Pharmacological Modification of a Cerebroplegia Solution
Published in Richard A. Jonas, Jane W. Newburger, Joseph J. Volpe, John W. Kirklin, Brain Injury and Pediatric Cardiac Surgery, 2019
Richard A. Jonas, Aoki Mitsuru
Twelve piglets (n = 7 for blood flow and metabolic study; n = 5 for MRS study) had 50 mL/kg of saline infused in the carotid artery system through the catheter in the subclavian artery at the initiation of hypothermic circulatory arrest. Doses of 10 mL/kg were repeated every 30 minutes during the hypothermic circulatory arrest (Group CPS). Eleven (n = 5 for blood flow and metabolic study; n = 6 for MRS study) received the same volumes of UW solution (Viaspan, Dupont Pharmaecuticals, Wilmington, DE) (Group CPU). Ten piglets (n = 5 for blood flow and metabolic study; n = 5 for MRS study) received the UW solution with 7.5 mg/L of MK-801 (Biochemical Research Laboratories, Natick, MA) (Group CPM). The dose of MK-801 was determined from calculated blood concentration in our previous study (see above) which explored systemic administration of MK-801 in a similar piglet model of hypothermic circulatory arrest. All solutions were infused at 4°C. Glutathione (3 mM/L reduced form, Sigma Chemical, St. Louis, MO) was added to the UW solution immediately prior to usage. The pH of the solutions was 6.30 ± 0.01(saline), 7.10 ± 0.02(UW), 7.00 ± 0.02(UW ± MK801). pO2 of the solutions ranged from 200 to 290 mmHg as measured at 37°C. The return effluent of the infused solution was drained and collected from a side arm from the venous cannula. Thirteen piglets (n = 7 for blood flow and metabolic study; n = 6 for MRS study) received no solution and served as controls.
Hair restoration surgery
Published in Jerry Shapiro, Nina Otberg, Hair Loss and Restoration, 2015
There are a variety of solutions that grafts can be stored in during the hair transplant procedure. Normal saline or Ringer’s Lactate are most commonly used as holding solutions. However, some physicians claim that more expensive solutions such as Hypothermosol, Viaspan, Wisconsin solution, or platelet-rich plasma are superior. To date, there is no ideal temperature or holding solution for hair restoration surgery based on current studies. Hair transplant data at this time suggest that chilled grafts in normal saline or Ringer’s Lactate seem to thrive just as well as when they are placed at room temperature in these solutions. It is uncertain whether it is better to use hypertonic solutions at a cool temperature, as is the case with other organs [35].
Adhesion characteristics of porcine pancreatic islets and exocrine tissue to coating materials
Published in Islets, 2018
Yoshiki Nakashima, Chika Miyagi-Shiohira, Naoya Kobayashi, Issei Saitoh, Masami Watanabe, Hirofumi Noguchi
University of Wisconsin solution (Viaspan) was obtained from Astellas Pharma Inc. (Tokyo, Japan). Fetal bovine serum (FBS) was obtained from BioWest (Nuaille, France). Iodixanol (OptiPrep) was obtained from AXIS-SHIELD PoC AS (Oslo, Norway). The Gradient Mixer and Ricordi Isolator Tubing Set were obtained from Biorep Technologies (Miami, FL, USA). Dulbecco's modified Eagle medium (DMEM), RPMI-1640 medium and 100 bp DNA Ladder One were obtained from Nacalai Tesque (Kyoto, Japan). Dithizone and gelatin from bovine skin were obtained from Sigma-Aldrich Co. LLC (St. Louis, MO, USA). Fibronectin Solution from Human Plasma was purchased from WAKO (Osaka, Japan). The plastic dishes were obtained from TPP (Trasadingen, Switzerland). All other materials used were of the highest commercial grade.