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The Modification of Histidine Residues
Published in Roger L. Lundblad, Chemical Reagents for Protein Modification, 2020
McCormick and co-workers42 have examined the reaction of diethylpyrocarbonate with pyridoxamine (pyridoxine)-5’-phosphate oxidase. The modification reaction was performed at pH 7.0 (0.1 M potassium phosphate containing 5% (v/v) EtOH) at 25°C generally in the presence of flavin mononucleotide (FMN). Figure 12 shows the time course for the modification of the oxidase under these experimental conditions. Also shown in Figure 12 is the dependence of the observed first-order rate constant on diethylpyrocarbonate concentration (panel B). The inset plot shows the reaction to be second-order with a rate constant of 12.5 M−1 s−1.
Edible Tuber Amorphophallus paeoniifolius (Dennst.) Extract Induces Apoptosis and Suppresses Migration of Breast Cancer Cells
Published in Nutrition and Cancer, 2021
Munmi Majumder, Manoj Sharma, Siddhartha Maiti, Rupak Mukhopadhyay
Identifying constituent compounds of an active extract is important to correlate association with anticancer effect. HR-LCMS analysis of the active ethyl acetate fraction of the extract showed the presence of five active compounds with reported anticancer activities. Among these compounds, Pyridoxine (Vitamin B6), in combination with other vitamins and zinc, showed anticancer activity against bladder cancer (13). The active form, pyridoxine 5′-phosphate, targeted pyridoxine 5′-phosphate oxidase and down regulated its expression in ovarian cancer cells leading to reduced proliferation (38). The flavonoid Cosmosiin induced apoptosis in stomach cancer cells and reduced risk of breast cancer (14). Fisetin, a well-known flavonoid, showed anticancer, and antimetastatic activity on various cancer cell lines like HL-60 (39), AsPC‐1 (40), SiHa and CaSki (41), HT-29 (42), MCF-7 (15). Plant stress hormone methyl jasmonate, also demonstrated antioxidant activity and inhibited metastasis and angiogenesis of cancer cells (16). Finally, dihydrogambogic acid showed potent anticancer activity against cell lines like MCF-7, HT-29, T47D (17).