Kidney microbiota dysbiosis contributes to the development of hypertension
Published in Gut Microbes, 2022
Xin-Yu Liu, Jing Li, Yamei Zhang, Luyun Fan, Yanli Xia, Yongyang Wu, Junru Chen, Xinyu Zhao, Qiannan Gao, Bing Xu, Chunlai Nie, Zhengyu Li, Aiping Tong, Wenjie Wang, Jun Cai
The presence of S. au was further confirmed with S. au-specific FISH probes and colocalized with sIgA (Fig. S7a). Visualization of isolated bacterial colonies after culturing for 4 weeks indicated cell wall disruption as a typical L-form morphology (Figure 1l). After a prolonged 8-month period of culturing and subculturing, which allowed the “fried eggs” L-form colonies to grow, the bacteria were approximately spherical, lacked cell walls, and showed vague boundaries (Figure 1n and Figure S7b). L-form bacteria grew when they returned to the normal structure with the bacterial cell wall, and TEM images in Figure 1n showed bacteria with impaired, incomplete, or intact cell walls, indicating the progress of L-form bacterial recovery.