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The Role of the Gut Microbiome in Cardiovascular Disease
Published in Stephen T. Sinatra, Mark C. Houston, Nutritional and Integrative Strategies in Cardiovascular Medicine, 2022
Pancreatic Elastase Treatment: Smoking cessationReduced alcohol consumptionSmall frequent mealsReplacement of fat-soluble vitaminsSupplemental lipase or pancreatic enzymes (plant-based digestive enzymes may not be strong enough for EPI)Prescription strength enzymes: CREON®, ZENPEP®, and others.
Enzyme-Releasing Peptide
Published in Jason Kelley, Cytokines of the Lung, 2022
Allen B. Cohen, Edmund J. Miller, Cassandra MacArthur
In an important study, Damiano and colleagues (1986) used immunolocalization techniques to identify elastase in sections of human lungs. They found elastase on elastin in areas of lungs with emphysema. These results have been confirmed by others (Yi-min et al., 1990). These studies come as close as possible, without carrying out a clinical trial of elastase inhibitors, to proving that neutrophil elastase causes some of the lung destruction in emphysematous lungs. In other studies in animals, Yamada and colleagues (1982) used morphometric methods to demonstrate that the neutrophils present in the lungs following cadmium injury were granule-poor.
Do I Have IBS?
Published in Melissa G. Hunt, Aaron T. Beck, Reclaim Your Life From IBS, 2022
Melissa G. Hunt, Aaron T. Beck
Exocrine pancreatic insufficiency (EPI) is a condition in which the pancreas doesn’t produce enough of the enzymes that help with certain aspects of digestion. Most people know that the pancreas makes insulin, which is crucial to managing glucose or blood sugar. But few people know that the pancreas also makes a number of digestive enzymes, including amylase (which breaks down carbohydrates), lipase (which breaks down fats), and protease and elastase (which break down proteins). If you can’t break down food, it passes through the intestines partially undigested, which can result in abdominal pain, gas, bloating, diarrhea (typically), or constipation, and, if it’s severe, weird poop that looks pale and oily, and can smell bad and sometimes floats (because there’s too much fat in it). One of the main causes of EPI is chronic pancreatitis, so if you’ve ever experienced even one incident of pancreatitis, it’s worth being tested for EPI. The easiest test for EPI is a stool test called the fecal elastase test (FE-1). Elastase is one of the digestive enzymes. If there is little or no elastase in your stool, that can indicate EPI.
Chemical composition, seasonal variation and antiaging activities of essential oil from Callistemon subulatus leaves growing in Egypt
Published in Journal of Enzyme Inhibition and Medicinal Chemistry, 2023
Omyma Rabie, Heba A. S. El-Nashar, Taghreed A. Majrashi, Tarfah Al‐Warhi, Mahmoud A. El Hassab, Wagdy M. Eldehna, Nada M. Mostafa
Elastin is a skin protein that plays a vital role in maintaining the healthy appearance and integrity of the skin15. Activation of elastase by ageing or exposure to UV rays and free radicals leads to the destruction of cutaneous elastin and thus loss of skin elasticity. The C. subulatus leaf oil of winter showed an inhibition to elastase activity with IC50 value of 1.05 ± 0.05 µg/mL, compared to the standard N-methoxysuccinyl-Ala-Ala-Pro-Val-chloromethyl ketone (IC50 = 0.55 ± 0.03 µg/mL). The essential oil of Illicium anisatum containing eucalyptol as a major component (36.7%) was able to inhibit elastase (IC50 = 1.79 mg/mL)35. Eucalyptol was one of the most abundant terpenes (11.82%) in Mentha viridis essential oil that showed elastase inhibition by IC50 = 114.24 ± 1.22 µg/mL36.
Anti-inflammatory effects of troxerutin are mediated through elastase inhibition
Published in Immunopharmacology and Immunotoxicology, 2020
Ramachandran Vidhya, Carani Venkatraman Anuradha
Troxerutin (TX), a derivative of bioflavonoid rutin has anti-obesity effect. TX is abundantly present in flower buds of Sophora japonica, tea, coffee, cereal grains and in a wide variety of fruits and vegetables [11]. Literature search reveals that TX also has antioxidant [12], anti-inflammatory [13], anti-diabetic [14] and anti-cancer properties [15]. Previous studies show that TX attenuates diet-induced oxidative stress and lipid changes in the heart of high-fat, high-fructose diet-fed mice [16]. We hypothesize that the anti-inflammatory properties of TX are mediated through inhibition of elastase. To verify this hypothesis, we investigated the effect of TX on elastase activity and the expression of adipokines in 3T3-L1 adipocytes treated with TNFα, an obesity-associated inflammatory cytokine. Elastatinal (ELAS), an elastase inhibitor [17] and sodium salicylate (SAL), a non-steroidal anti-inflammatory drug [18] were included in the study for comparison. The elastase inhibitory potential of TX was assessed by in vitro enzyme assay. Molecular docking analysis of HNE crystal structure (PDB ID: 3Q76) with TX and ELAS was done using Schrödinger maestro suite of Glide [19] for comparing the binding properties.
Methodological evaluation of gingival crevicular fluid volume and neutrophil elastase levels: sequential sampling, length of sampling time and two different sampling methods
Published in Acta Odontologica Scandinavica, 2020
Nezahat Arzu Kayar, Bahar Füsun Oduncuoğlu, Seyfullah Haliloğlu, Behiç Serpek, Tamer Ataoğlu, Nilgün Özlem Alptekin
At the time of analysis, samples were vortexed and incubated at 1 h at 22 °C. On each microtiter plate, 25 μl of elution media (physiologic saline ± 0.1% Tween 20) and 200 μl of substrate was added into two wells in the first column as the substrate blank. Duplicate 25 μl samples of each standard and 200 μl of substrate was placed into standard wells. A total of 25 μl of sample and 200 μl of substrate was added to sample wells. A 25 ml aliquot of GCF was incubated with 200 ml of 1.25 mM synthetic substrate specific for NE, N-methoxysuccinyl-ala-ala-pro-val-p-nitroanilide (Sigma Chem. Co., St Louis) in a 96-well ELISA plate for 2.5 h at 37 °C. At the end of the incubation, the optic density was read at 405 nm. This method measures functionally active NE, which includes free NE and NE bound to a2-macroglobulin (A2MG). Elastase-a1-antitrypsin (A1AT) inhibitory complex was not recorded by this assay since it was functionally inactive [47]. Active NE levels (free elastase and elastase bound to A2MG) were calculated from the standard curve. NE activity on the strips was expressed in terms of µU (pmols of substrate hydrolysed per minute) and μU/μl [48,49].