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A Review on L-Asparaginase
Published in Se-Kwon Kim, Marine Biochemistry, 2023
Collagenase aids in the treatment of burns and skin ulcers. It helps to lyse and remove dead skin and dead tissue, thereby helping the repair mechanism. This ultimately improves the action of antibiotics to work better in improving the individual’s healing process (Ostlie et al., 2012). Lipase helps in the treatment of digestive disorders. By activating the tumor necrosis factor, they can be used in the treatment of malignant tumors. Disorders like dyspepsia, gastrointestinal disturbances and digestive allergies are treated with lipase enzymes. Lipase obtained from Candida rugosa is used to produce lovastatin, a drug that has the ability to decrease serum-level cholesterol. The hydrolysis of 3-phenylglycidic acid ester is a key intermediate in the production of diltiazem hydrochloride. It is more commonly used as a coronary vasodilator and is synthesized from Serratia marcescens lipase.
Biotransformation of Sesquiterpenoids, Ionones, Damascones, Adamantanes, and Aromatic Compounds by Green Algae, Fungi, and Mammals
Published in K. Hüsnü Can Başer, Gerhard Buchbauer, Handbook of Essential Oils, 2020
Yoshinori Asakawa, Yoshiaki Noma
The same substrate was incubated in A. niger, Aspergillus oryzae, Candida rugosa, Candida tropicalis, Mucor mucedo, Bacillus subtilis, and Schizosaccharomyces pombe; however, any metabolites have been obtained. All microbes except for the last organism, zerumbone epoxide (409), prepared by mCPBA, bioconverted into two diastereoisomers, 2R,6S,7S-dihydro- (411) and 2R,6R,7R-derivative (412), whose ratio was determined by GC, and their enantio-excess was over 99% (Nishida and Kawai, 2007) (Figure 23.119).
Alternative method to improve the ethyl valerate yield using an immobilised Burkholderia cepacia lipase
Published in Journal of Microencapsulation, 2019
Wellington Correa Moreira, Alfredo Luís Pereira Elias, Wislei Riuper Osório, Giovana Silva Padilha
From the results, it can be observed that the initial activity and recovered activity of the lipase are 442 ± 5U/g and 430 ± 3U/g, respectively. It is worth noting that the non-buffer pH provides an activity of about 386 ± 5U/g, which implies a decreasing approximately 11%. Besides, an encapsulation efficient of 81.8 ± 3% (w/w) is reached, lower than that the value shown in Table 1. The averages of all examined buffer pH values are considered. The highest ethyl valerate yield is that of Burkholderia cepacia into the alginate with a buffered pH. This study provides an efficient method to enhance the using dehydrated immobilised lipase from Burkholderia cepacia. The buffer pH system is a reference/control considering future studies when esterification reaction is taken in account. Considering 3h, the buffered medium is of about 2 times higher than a non-buffered medium. With this, the interaction between the buffered pH and the alginate-lipase has an important role on the increase of the ethyl valerate yield. It is also worth noting that the GC analyses confirm these mentioned results. In previous studies developed by Betigeri and Neau (2002), an immobilised Candida rugosa lipase on the polymers such as agarose, alginate and chitosan was investigated. The lipase can be successfully immobilised by using both the alginate and chitosan gel beads. Considering the fresh and freeze-dried beads, the activity in the alginate was lower than the chitosan. The agarose beads have demonstrated certain susceptibilities to swelling and to disintegrate in the test medium examined. Cheirsilp et al. (2009) have produced monoacylglycerol (MAG) from the palm oil with the addition of the glycerol Pseudomonas sp in the alginate, and the MAG yield has been increased. Ozyilmaz and Geser (2010) have obtained the ethyl valerate, isoamyl acetate and the butyl acetate using Candida rugosa lipase (CLR) and a porcine pancreatic lipase (PPL) entrapped in a calcium alginate gel. For both the CRL and PPL, the maximum production of the ester between 40 °C and 50 °C was reached. The highest ester yield was that of the isoamyl acetate when the lipase onto the PPL was immobilised.