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Anticancer Properties of Silver Nanoparticles from Root Extract of Trigonella Foenum-Graecum
Published in Megh R. Goyal, Preeti Birwal, Santosh K. Mishra, Phytochemicals and Medicinal Plants in Food Design, 2022
Ramasamy Harikrishnan, Lourthu Samy S. Mary, Gunapathy Devi, Chellam Balasundaram
TFAgNPs showed peaks at 1653.61 cm−1 and the bonds due to O–H extending of phenol constituent [47]; whereas the remaining peaks acquired in TFAgNPs sample are 3870.00 and 3907.08 cm−1 due to O=H extending of –OH groups [5]. The stretch at 2925.817 cm−1 was identified and it signifies –C–H stretching [57]. The intense peak of 539.08 cm−1 shows C–Br group of alkyl halides, whereas the peak at 2925.81 cm−1 suggest the occurrence of N–H bend, which demonstrating as secondary amine groups present in the protein. Likewise, 1651 cm−1 band resemble to the primary amine groups such as N–H bending or carbonyl extending sensations of protein have been detected [50].
The Modification of Cysteine
Published in Roger L. Lundblad, Chemical Reagents for Protein Modification, 2020
Dahl and McKinley-McKee21 have made a rather detailed study of the reaction of alkyl halides with thiols. It is emphasized that reactivity of alkyl halides not only depends on the halogen but also on the nature of the alkyl groups. These investigators emphasized that the reactivity of an alkyl halide such as iodoacetate depends not only on the leaving potential of the halide substituent (I > Br >>> CI; 130:90:1) but also on the nature of the alkyl group. The rate of reaction of 2-bromoethanol with the sulfydryl group of L-cysteine (pH 9.0) is approximately 1000 times less than that observed with bromoacetic acid. The reactions are extremely pH dependent, emphasizing the importance of the thiolate anion in the reaction.
Halogen Labeled Compounds (F, Br, At, Cl) *
Published in Garimella V. S. Rayudu, Lelio G. Colombetti, Radiotracers for Medical Applications, 2019
The halogen exchange reaction could also be accomplished on gas chromatographic columns to prepare some volatile 82Br- and 18F-labeled alkyl halides as discussed previously (Section III.C.3).139, 140
Semisynthesis and anti-cancer properties of novel honokiol derivatives in human nasopharyngeal carcinoma CNE-2Z cells
Published in Journal of Enzyme Inhibition and Medicinal Chemistry, 2023
Bo-Han Li, Hui Ma, Jing Zhu, Jie Chen, Yi-Qun Dai, Xiao-Jing Zhang, Hong-Mei Li, Cheng-Zhu Wu
As shown in Scheme 1, a series of HNK derivatives were designed and synthesised using honokiol as the starting compound. Compounds 1a–1c, 2a–2c, and 3a–3c were synthesised from HNK through Williamson alkylation of alkyl halides. Next, compounds 1d–3d and 1e–3e were synthesised via C-formylation using NaOH/tetrabutylammonium bromide (TBAB) in CHCl3 as the catalyst. The latter compounds 1d–3d and 1e–3e were further reacted with aminoguanidine bicarbonate in the presence of catalytic amounts of hydrochloric acid to provide 1f–3f and 1g–3g, respectively. The chemical structures of all compounds were characterised by 1H-NMR, 13C-NMR, and HR-ESI-MS. The detailed synthesis processes and an overview of the physical and analytical data were described in the experimental section (see Supplementary Materials).
Design, synthesis, and evaluation of novel O-alkyl ferulamide derivatives as multifunctional ligands for treating Alzheimer’s disease
Published in Journal of Enzyme Inhibition and Medicinal Chemistry, 2022
Gaofeng Zhu, Ping Bai, Keren Wang, Jing Mi, Jing Yang, Jiaqi Hu, Yujuan Ban, Ran Xu, Rui Chen, Changning Wang, Lei Tang, Zhipei Sang
Alkyl halide 4a–4d (1.3 mmol) was added to a mixture of anhydrous K2CO3 (1.2 mmol), and the key intermediate 3a–3c (1.0 mmol) in 8 ml anhydrous CH3CN. The reaction mixture was heated to 65 °C and stirred for 6–10 h under an argon atmosphere. The reaction was monitored by TLC. On completion of the reaction, the solvent was evaporated under reduced pressure. The crude residue was treated with 30 ml of water and the mixture was extracted with CH2Cl2 (2 × 30 ml). The combined organic phases were washed with saturated NaCl (50 ml), dried over anhydrous Na2SO4, and filtered. The solvent was evaporated under reduced pressure and the residue was purified by silica gel chromatography (petroleum ether/acetone = 50:1) to get compounds 5a–5i.
The SNAP-tag technology revised: an effective chemo-enzymatic approach by using a universal azide-based substrate
Published in Journal of Enzyme Inhibition and Medicinal Chemistry, 2021
Rosa Merlo, Diego Caprioglio, Michele Cillo, Anna Valenti, Rosanna Mattossovich, Castrese Morrone, Alberto Massarotti, Franca Rossi, Riccardo Miggiano, Antonio Leonardi, Alberto Minassi, Giuseppe Perugino
The advent of the self-labelling protein-tags (SLPs) has led to a huge push in modern biotechnology, especially in the field of cell biology, where auto-fluorescent proteins (AFPs) for a long time dominated for their versatility in the localisation experiments of proteins, organelles, and membranes1. But the use of SLPs clearly goes beyond: they catalyse the covalent, highly specific and irreversible attachment of a part of their synthetic ligands upon reaction. This offers the opportunity to label them by conjugation to those ligands of an infinite number of chemical groups, such as fluorescent dyes, affinity molecules, or solid surfaces, expanding the application fields2. Among SLPs, of particular note are the Halotag®, the SpyTag3 the SNAP- and the CLIP-tag®. The Promega Halotag® is a halo-alkane dehalogenase with a genetically modified active site, which reacts irreversibly with primary alkyl-halides4,5.