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Interactive Textiles for Well-being in Dementia-friendly Communities
Published in Paul A. Rodgers, Design for People Living with Dementia, 2022
Lucy Robertson, Chris Lim, Wendy Moncur
Three initial workshops were conducted:An Introduction to E-textilesPrinting with Heat Reactive DyesPrinting with Light
Application of Genomic, Proteomic, and Metabolomic Technologies to the Development of Countermeasures against Chemical Warfare Agents
Published in Brian J. Lukey, James A. Romano, Salem Harry, Chemical Warfare Agents, 2019
Jennifer W. Sekowski, James F. Dillman III
Early CWA detectors were quite primitive and included primarily chemical-reactive dyes in paints. These were insensitive and therefore unreliable (Smart, 1997). Later technology used more sensitive chemical dyes in other formats such as paper tickets. Modern detectors now consist of fieldable IR spectrometers and an alarm system designed to warn of the presence of CWAs on the battlefield or in an enclosed space. Several reliable tests for the diagnosis of CWA exposure have been developed. To detect HD exposure, the level of thiodiglycol, a metabolite of HD, is quantitated in the urine using a gas chromatography-mass spectrometry (GC-MS) analytical method (Jakubowski et al., 1990; TB MED 296, 1996). Nerve agent exposure is detected in the field by the use of a fieldable Ellman assay to determine cholinesterase inhibition in the blood (Ellman et al., 1961; TB MED 296, 1996).
Contact Urticaria Syndrome from Reactive Dyes in Textiles
Published in Ana M. Giménez-Arnau, Howard I. Maibach, Contact Urticaria Syndrome, 2014
Dyes are referred to either through their trade name or Color Index name, whereas the chemical class and to a greater extent the reactive group not always is known.[1] In a publication from Romano et al. from 1992, an extensive table lists the RDs described until then in the literature as a cause of allergic reactions in humans.[1] Besides RD molecules, commercial reactive dye stuffs also contain several other components to improve their dyeing properties, and many of these may also be sensitizers. In Finland, Estlander tried to patch test with scrapings from a thin-layer chromatography plate in which a RD called Remazol Schwarz B had given 10 spots, but the patch tests were negative.[2]
Characterization of a novel anti-human lymphocyte activation gene 3 (LAG-3) antibody for cancer immunotherapy
Published in mAbs, 2019
Xiaojie Yu, Xiao Huang, Xiuxiu Chen, Jianfei Liu, Chenglin Wu, Qian Pu, Yuxiao Wang, Xiaoqiang Kang, Lijun Zhou
Endocytosis of antibodies was determined using Jurkat-NFAT-LAG-3 and HEK293-LAG-3 cells. In particular, LBL-007 and relatlimab analog were first conjugated with pHAb Reactive Dye (G9845, Promega) following the manual instructions. The pHAb Reactive Dye is a hydrophilic bright pH-sensor dye that becomes fluorescent at acidic pH, and can be used for high-throughput antibody internalization screening assays. Upon receptor-mediated internalization, antibody-pHAb conjugates track through the endosomal and lysosomal systems, the pH drops, and dyes become highly fluorescent.20,38 Antibody concentration and dye-to-antibody ratio were calculated as follows: antibody concentration (mg/ml) = A280-(A532 × 0.256)/1.4; and the dye-to-antibody ratio = A532 × 150000/Ab concentration×75000. The cells were incubated with the dye-conjugated LBL-007 and relatlimab analog at 10 µg/mL per 1 × 105 cells at 37°C for 0.5, 2, 5, or 24 h. After two washes with PBS, the cells were collected by centrifugation and the fluorescence signal was measured by flow cytometry.
HLA ligandome analysis of primary chronic lymphocytic leukemia (CLL) cells under lenalidomide treatment confirms the suitability of lenalidomide for combination with T-cell-based immunotherapy
Published in OncoImmunology, 2018
Annika Nelde, Daniel J. Kowalewski, Linus Backert, Heiko Schuster, Jan-Ole Werner, Reinhild Klein, Oliver Kohlbacher, Lothar Kanz, Helmut R. Salih, Hans-Georg Rammensee, Stefan Stevanović, Juliane S. Walz
HLA surface expression on CD19+CD5+ CLL cells and CD19+CD5− autologous B cells of CLL patients was analyzed using the QIFIKIT bead-based quantitative flow cytometric assay (Dako, K0078) according to manufacturer's instructions as described before.53 In brief, samples were stained with the pan-HLA class I-specific monoclonal antibody (mAb) W6/32 (produced in-house), the HLA-DR-specific mAB L243 (produced in-house) or IgG isotype control (BioLegend, 400202), respectively. Surface marker staining was performed with directly labeled APC anti-human CD19 (BioLegend, 302212), PE anti-human CD5 (BioLegend, 300608) and APC-H7 anti-human CD3 (BD, 641406) antibodies. Aqua fluorescent reactive dye (life technologies, L34957) was used as viability marker. Flow cytometric analysis was performed on a FACSCanto II Analyzer (BD).
Effects of MDMA (ecstasy) on apoptosis and heat shock protein (HSP70) expression in adult rat testis
Published in Toxicology Mechanisms and Methods, 2018
Fahimeh Mobaraki, Masoumeh Seghatoleslam, Alireza Fazel, Alireza Ebrahimzadeh-Bideskan
For this technique, after deparaffinization and rehydration of the sections, the endogenous peroxidase activity was blocked by 3.0% hydrogen peroxide for 20 min at 37 °C. The samples were then washed three times with PBS and were subjected to 10% normal goat serum. Next, the sections were incubated with the anti-HSP70 (Abcam Co., Cambridge, MA; Cas. No. 109689) as a primary antibody at 4 °C overnight and after washing with PBS, they incubated with anti-rabbit IgG peroxidase conjugate (Abcam Co., Cambridge, MA; Cas. No. 97051) as a secondary antibody for 45 min at ambient temperature. Finally, the samples were immersed in DAB solution for 10–15 min at room temperature in dark. At the end, after imaging of tissue sections, numerical density of cells labeled with antibodies against HSP70 was calculated per unit area using grades containing unbiased frames according to the above mentioned method and formula. Three people investigated the prepared samples with an optical microscope (Olympus, model Bx50, Tokyo, Japan) to assess the severity of reactive dye by the blind method and the intensity of reaction was determined for each sample using Likert spectrum (no reaction= 0, poor reaction= +, moderate reaction= ++, severe reaction= +++ and very severe reaction= ++++). The data obtained from different groups were compared using Kruskal–Wallis test and SPSS software (SPSS Inc., Chicago, IL) (Mohammadi et al. 2013).