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A novel probiotic, Lactobacillus johnsonii 456, resists acid and can persist in the human gut beyond the initial ingestion period
Published in Gut Microbes, 2019
Michael J. Davoren, Jared Liu, Jocelyn Castellanos, Norma I. Rodríguez-Malavé, Robert H. Schiestl
Bile acid tolerance was evaluated using a modified version of the method of Gilliland and Walker.99 Each probiotic strain was evaluated based on addition of bile salts to their standard growth conditions. Freshly inoculated culture media was vortexed heavily and then split evenly into either a fresh vial or one containing ox gall extract (Sigma) to 0.1, 0.2, or 0.3% of final solution by weight (2.12, 4.24, and 6.36 mM based on rough Sigma ox gall extract bile acid salt composition: 10% glycocholic acid, 15% glycodeoxycholic acid, 30% taurocholic acid, 55% cholic acid) Culture media was then incubated anaerobically for 18–20 hours at 37° C, and samples were plated and enumerated.