China
Africa
Explore chapters and articles related to this topic
Role of Histone Methyltransferase in Breast Cancer
Published in Meenu Gupta, Rachna Jain, Arun Solanki, Fadi Al-Turjman, Cancer Prediction for Industrial IoT 4.0: A Machine Learning Perspective, 2021
Surekha Manhas, Zaved Ahmed Khan
The effectiveness of G9a inhibitors or DNMT inhibitors is easily recognizable in cellular-based activities for in in vitro studies. MTT assay has been used in different research approaches to evaluate drug or inhibitor effectiveness. In addition, the anti-tumor activity of different drugs is predictable by targeting the methyltransferase activity of G9a and DNMTs through MTS assay.
The Evolution of Anticancer Therapies
Published in David E. Thurston, Ilona Pysz, Chemistry and Pharmacology of Anticancer Drugs, 2021
Cell-based screens are also carried out by some companies, although this requires more complex equipment due to the need to control the temperature, gaseous environment, and sterility to ensure satisfactory cell growth. For example, tumor cells can be grown in multi-well plates, and individual compounds added to each well. A colorimetric detection method such as the MTT assay (Figure 2.7) can then be used to measure the number of dead versus living cells. This assay is based on the reduction of a yellow tetrazolium salt (MTT; 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) by NAD(P)H-dependent oxidoreductase enzymes to purple formazan crystals by metabolically active cells. A. The cell-based MTT assay is based on conversion of the yellow MTT compound to the purple formazan via NAD(P)H-dependent oxidoreductase enzymes. B. A 96-well plate showing no effect on the cells in 92 of the wells, whereas the compounds in four wells on the left-hand side of the plate have killed the cells (i.e., the cells are not metabolizing the MTT).
Essential Oils in Cancer Therapy
Published in K. Hüsnü Can Başer, Gerhard Buchbauer, Handbook of Essential Oils, 2020
Carmen Trummer, Gerhard Buchbauer
Navel orange is a type of citrus which is cultivated in many countries around the world and Gannan in Jiangxi Province is the top navel orange producing area in China (Eldahshan and Halim, 2016). Yang et al. (2017) studied the EO from Gannan navel orange peel, with 74.6% of limonene, for its anticancer activities. The EO was able to inhibit the proliferation of a human lung cancer cell line A549 and prostate cancer cell line 22RV-1. They used the MTT assay to evaluate the effect of different concentrations on cell viability. Concentrations between 6.25 and 200 μg/mL showed the best inhibition of proliferation in both cell lines. The higher the concentration of the navel orange EO, the better was the inhibition.
Altered VDAC-HK association and apoptosis in mouse peripheral blood lymphocytes exposed to diabetic condition: an in vitro and in vivo study
Published in Archives of Physiology and Biochemistry, 2023
Melinda Nongbet Sohlang, Suktilang Majaw
MTT assay is a sensitive indicator of the metabolic viability indicative of mitochondrial function (Fiorillo et al. 2006). As shown in Figure 3(a), decreased mitochondrial activity (p < .001) was observed in PBL exposed to high Glc/PA as well as in that of diabetic PBL with p < .001. Changes in MMP directly contribute to mitochondria-mediated apoptosis. This change in MMP was analysed using JC-1 dye. Healthy cells with high MMP have JC-1 crossing the plasma membrane thereby forming aggregates that emit signal mostly at the red fluorescent channel while apoptotic cells have lower MMP where JC-1 remains in the cytoplasm as a monomeric form emitting a signal at the green channel. A lower MMP was observed in PBL treated with high Glc/PA and diabetic PBL (p < .001) compared to control groups (Figure 4(a)) as seen by the higher percentages of cells in the green fluorescence region (Figure 4(b)). This may therefore result in the release of apoptotic factors that initiate apoptosis by activating the death caspase indicated by caspase-3 (Porter and Janicke 1999).
New benzoxazole derivatives as potential VEGFR-2 inhibitors and apoptosis inducers: design, synthesis, anti-proliferative evaluation, flowcytometric analysis, and in silico studies
Published in Journal of Enzyme Inhibition and Medicinal Chemistry, 2022
Hazem Elkady, Alaa Elwan, Hesham A. El-Mahdy, Ahmed S. Doghish, Ahmed Ismail, Mohammed S. Taghour, Eslam B. Elkaeed, Ibrahim H. Eissa, Mohammed A. Dahab, Hazem A. Mahdy, Mohamed M. Khalifa
The anti-proliferative activity of all tested compounds was performed on MCF-7 and HepG2 cells by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay35,41–44. The MTT assay is based on the capability of living cells to reduce the yellow product MTT to a blue product, formazan, by a reduction reaction occurring in the mitochondria. Briefly, in MTT assay, 5000 cells/well were plated in a 96-well plate and allowed to grow 24 h, then treated with Roswell Park Memorial Institute (RPMI) 1640 media that contain increased concentrations (0, 0.1, 1, 10, 100, and 1000 µM) of tested compounds. Each experiment was carried out in triplicate. Then media were removed and 100 µL of MTT was added to each well and incubated for 4 h. The formed formazan crystals were solubilised by adding 100 µL of dimethyl sulfoxide (DMSO) solution and absorbance was measured at 570 nm using ELISA microplate reader (Epoc-2 C micro-plate reader, Bio Tek, VT, USA). The IC50 values [the concentration required for 50% inhibition of cell viability] were calculated and the results are expressed as the relative percentage of the control cells (100% of cell viability).
The combination of MnO2@Lipo-coated gefitinib and bevacizumab inhibits the development of non-small cell lung cancer
Published in Drug Delivery, 2022
Jisong Zhang, Li Xu, Huihui Hu, Enguo Chen
MTT assay: Cell viability in the experimental and control groups was assessed by MTT assay. A549 and 16HBE cells with densities of 5 × 104 cells/mL were inoculated in 96-well plates (containing 100 μL medium/well), and incubated at 37 °C with 5% CO2 overnight. Then, the medium was removed and the cells were suspended in fresh mediums (containing free Geb, MnO2-PDA@Lipo@Geb, MnO2-PDA@Lipo@Geb@Beb), with concentrations of Geb of 0, 1, 5, and 10 μg/mL, respectively. At the same time, the cells treated by medium alone were set as the control group. After that, the plates with different treatments were incubated at 37 °C with 5% CO2 overnight for 24 h. Afterwards, 20 μL MTT solution (5 mg/mL) was added to each well dropwise and incubated for 4 h. The original medium was removed completely and 150 μL dimethyl sulfoxide (DMSO) was added. Finally, the OD value at the wavelength of 450 nm was detected by a microplate reader (Victor X, PerkinElmer, Waltham, MA).