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Host-Parasite Interactions With Macrophages In Culture
Published in Hans H. Gadebusch, Phagocytes and Cellular Immunity, 2020
Lee S. F. Soderberg, Morris Solotorovsky
Macrophage lipids may be characterized by histochemical stains or by incorporation of radiolabeled precursors. A variety of histochemical stains for lipids has been employed in the study of macrophages. Macrophages have been stained for lipid with Sudan black B stain59,110 and for phospholipids using Baker’s acid hematein59 and methasol fast-blue stain.110 Macrophages have been stained for neutral fats with oil red O.110 However, stains do not measure metabolic activity, but precursors may be included in the maintenance medium. Lipid precursors have included 3H-choline,9714Clecithin, and 14C-lysolecithin.111 Labeled choline was incorporated into membrane lecithin.97 Macrophage metabolism of 14C-lysolecithin into lecithin could be monitored. Then cephalin, lecithin, spingomyelin, and free fatty acids could be isolated by chromatography.
Pathology—Patient with possible Hirschsprung disease: Case study
Published in Victoria A. Lane, Richard J. Wood, Carlos A. Reck-Burneo, Marc A. Levitt, Pediatric Colorectal and Pelvic Surgery, 2017
Victoria A. Lane, Richard J. Wood, Carlos A. Reck-Burneo, Marc A. Levitt
H + E stain: One of the principal stains in histology.Staining involves the application of hemalum, a complex formed from aluminum ions and hematein (an oxidation product of hematoxylin).Hemalum stains nuclei blue.The nuclear stain is followed by counterstaining with eosin, which colors other structures red/pink/orange.H + E staining remains the method of choice for the identification of ganglion cells.Regular biopsies for H + E slide studies require fixation of material in buffered formalin and then standard processing.
Treatment Of Lightning Injury
Published in Christopher J. Andrews, Mary Ann Cooper, Mat Darveniza, David Mackerras, Lightning Injuries: Electrical, Medical, and Legal Aspects Editors, 1992
Christopher J. Andrews, Mary Ann Cooper, Mat Darveniza, David Mackerras
Both myoglobinuria4,5,8,20,35 and hemoglobinuria4,8,20 have been recorded following lightning strike. Unfortunately, their severity is difficult to estimate from published reports. Modest amounts of myoglobin or acid hematein are rapidly excreted by the kidney; however, massive amounts may cause acute renal failure. Excretion of myoglobin is enhanced by increasing urine volume and the maintenance of an alkaline urine. The use of mannitol and intravenous sodium bicarbonate is protective.45 However, increased urinary losses must be replaced and renal perfusión maintained. Attention should also be directed toward the provision of adequate urinary drainage, the prevention of urinary tract infection, and maintenance of adequate renal perfusión.
Haematoxylin – the story of the blues
Published in British Journal of Biomedical Science, 2018
Prior to this time, during the Elizabethan era, early fabric dyers in England found the colours of haematoxylin to be ‘fugitive’. Even colourisation of hands and shirt collars was readily removed from those working with the dye. As a result and due to the dye’s lack of permanency, an Elizabethan prohibition followed which lasted nearly a century. This paved the way for the introduction of the mordant, which imparted a long-lasting permanency for the dye. The extracted haematoxylin was extracted and subsequently oxidised in boiling water to form hematein. The hematein is a complex phenolic compound similar to flavonoid pigments of flowers. There are two basic procedures which convert the haematoxylin to hematein, natural oxidation (‘ripening’) by exposure to light and air or alternatively chemical oxidation employing either sodium iodate or mercuric oxide and potassium permanganate. The chemical method is much faster and results in instantaneous oxidation.