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Tours of St Vincent, Trinidad and Grenada (September–December 1901)
Published in Gordon C Cook, Caribbean Diseases, 2018
I got back here from Demerara (British Guiana) last week. After arriving I began to feel seedy with aching in my bones and eventually developed malignant malarial fever [P. falciparum infection]. With energetic treatment by quinine I completely had it under in two days and though tiring easily am all right again. What I disliked most was the enormous perspiration, the weather also at the time being about 90°in the shade. It was interesting in a way. Calculating back I found that 10 days before that I had been at Morawhanna [seeFigure 6.1] a frightfully unhealthy place in the N.W. of B. Guiana. Most of the people there had suffered from bad fever, and as I slept there in a hammock which I could not properly protect by a mosquito net the sequence of events referable to anopheles bite is evident. I am glad it had not knocked me up for long as I am going to St. Vincent the day after tomorrow again to pursue Demarquaii [sic]. It was interesting to receive your letter with the note, or reprint rather, about sleeping sickness and of my paper on Barbados. I got these the day after coming back and I also got the xylol, the fuchsine [sic] and the [?] a day or so ago.
Microwave assisted green synthesis of silver nanoparticles for optical, catalytic, biological and electrochemical applications
Published in Artificial Cells, Nanomedicine, and Biotechnology, 2021
V. N. Anjana, Majo Joseph, Sijo Francis, Alex Joseph, Ebey P. Koshy, Beena Mathew
The catalytic activity of plant derived AgNP was investigated using the degradation reactions of fuchsine and methylene blue using NaBH4. These dyes were selected for our study because its absorption maximum does not overlap with the SPR band of AgNPs [26]. Fuchsine or rosaniline hydrochloride is a magenta dye with chemical formula C20H19N3·HCl. The UV–vis absorption spectrum of an aqueous solution of fuchsine shows peaks at 292 nm and 546 nm. The reduction of fuchsine into leucofuchsine can be followed spectrophotometrically by monitoring the absorption maximum at 546 nm. Comparing Figure 5(a,b), it is clear that when AgNP was added to the reaction mixture containing both fuchsine and NaBH4, the intensity of the peak at 546 nm began to decrease continuously with the passage of time. The kinetic studies were performed by ln[A] versus time graph (Figure 5(c)). Rate constant determination proved a pseudo-first order kinetics with respect to the concentration of the dye [64].
Glial-derived neurotrophic factor is essential for blood-nerve barrier functional recovery in an experimental murine model of traumatic peripheral neuropathy
Published in Tissue Barriers, 2018
Chaoling Dong, E. Scott Helton, Ping Zhou, Xuan Ouyang, Xavier d‘Anglemont de Tassigny, Alberto Pascual, José López-Barneo, Eroboghene E. Ubogu
Plastic-embedded sciatic nerve blocks were trimmed and axially sectioned with an LKB Ultrotome III ultramicrotome using a DiATOME Histo diamond knife. 0.5-1.0 µm semi-thin sections were mounted on glass slides and stained with basic fuchsin, followed by methylene blue counterstain. Briefly, 2–3 drops of basic fuchsin (Rosalinine chloride, basic violet 14 powder; 0.1 mg/mL in 30% ethanol) were applied to each glass slide to completely cover the sections. Slides were heated on a hot plate at ∼90–95°C for 15 seconds. Excessive stain was washed from each slide with a stream of distilled water. The methylene blue counterstain was performed by covering basic fuchsin-stained sections with 1–2 drops of methylene blue (0.6 mg/mL in 2% aqueous sodium tetraborate). Slides were heated on a hot plate at ∼90-95°C for 10 seconds followed by a wash with a stream of distilled water. Each slide was air-dried and mounted with coverslips using Cytoseal 60 mounting medium prior to taking digital photomicrographs using a Nikon DS-Fi2 color camera mounted on a Leitz Laborlux S light microscope. Images were processed using the Nikon Elements software.
A novel CRYGC E128* mutation underlying an autosomal dominant nuclear cataract in a south Indian kindred
Published in Ophthalmic Genetics, 2020
Dinesh Kumar Kandaswamy, K. Vasantha, Jochen Graw, Sathiyaveedu Thyagarajan Santhiya
CRYGC wild-type clones were created using pCR™2.1-TOPO vector (Thermo Fisher Scientific, Darmstadt, Germany), following the manufacture’s protocol for PCR-based cloning. CRYGC mRNA was amplified from cDNA of human lenticular extract of a patient’s sample without CRYGC mutation using custom designed primers (Sequence available upon request). Mutant clones were created using GeneArt® Site-Directed Mutagenesis PLUS System (Thermo Fisher Scientific), following the manufacture’s protocol using mutant primers (Sequence available upon request). The clones were verified by sequencing for correct orientation. The 5′ capped mRNA of both wild-type and mutants were synthesized from pCR™2.1-TOPO-CRYGC using mMESSAGE mMACHINE T7 kit (Thermo Fisher Scientific), purified using RNeasy Mini kit (Qiagen, Germany) and quantified using NanoDrop (Thermo Fisher Scientific). About 50–100 pg of RNA dissolved in RNA free water was injected into zebrafish embryos at the one-cell stage. For manual examination, zebra fish embryos were cultured in 0.003% PTU (1-phenyl-2-thiourea, Sigma, Germany) after 24 hpf to prevent pigment formation, as it could affect the documentation and anesthetized in tricaine at 24 hpf for documentation. Live embryos were observed under a stereo microscope ((Leica MZ10F, Germany). At 3 days post fertilization (dpf), both embryos (n = 3 in each group wild-type and mutant mRNA injected embryos) were fixed in PFA and embedded in Technovit. Sections were taken and stained with methylene blue (0.13%) and basic fuchsin (0.13%) (Sigma-Aldrich, Steinheim, Germany). The stained sections were photographed under light microscope (Axioplan, Zeiss, Oberkochen, Germany).