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Preservative Resistance
Published in Philip A. Geis, Cosmetic Microbiology, 2020
Strains of Pseudomonas putida and Alcaligenes xylosoxidans ssp. denitrificans have been reported to exhibit high levels of triclosan resistance due to each of these two bacterial species being able to degrade triclosan by using enzymes (37). Triclosan degradation has also been demonstrated in Sphingomonas strain RD1 by the release of 14CO2 from 14C-labeled triclosan (38). Two root fungi, Trametes versicolor and Pycnoporus cinnabarinus, have been shown to transform triclosan by either glucosylation or xylosylation of the hydroxyl group (39).
Aquatic Plants Native to Europe
Published in Namrita Lall, Aquatic Plants, 2020
Isa A. Lambrechts, Lydia Gibango, Antonios Chrysargyris, Nikolaos Tzortzakis, Namrita Lall
When different extracts of the plant were tested against 26 strains of bacteria and fungi, it was reported that the activity varied depending on the type of plant extract and on the group of microorganisms (Radojevic et al. 2016). It has been reported that the ethanolic extract of the plant had high activity against Proteus mirabilis, even higher than that of the positive control (tetracycline). In general, Gram-positive bacteria were more sensitive to the extracts of the plant. The antifungal activity of the plant extracts was relatively low, except for the activity against Aspergillus restrictus, where the minimum inhibitory concentrations were found to be better than the control (fluconazole). Another report demonstrated the antimicrobial activity of the plant against Pseudomonas putida, P. morganii, P. mirabilis, and K. pneumoniae (Parekh and Chanda 2007). It has been mentioned that when different solvents were used for the antimicrobial tests, the activity increased with increasing polarity of the solvent.
Enzymatic Amino Acid Deprivation Therapies Targeting Cancer
Published in Peter Grunwald, Pharmaceutical Biocatalysis, 2019
Carla S. S. Teixeira, Henrique S. Fernandes, Sérgio F. Sousa, Nuno M. F. S. A. Cerqueira
MGL has a broad range of application in cancer therapies. Since this enzyme is absent in mammals, MGL was initially isolated from Clostridium sporogene (Sharma et al., 2014). Later on, the enzyme from Pseudomonas putida was chosen for clinical purposes, due to its greater stability and affinity for the substrate (low KM) (Esaki and, Soda, 1987). Therefore, the MGL gene from P. putida was isolated and expressed in Escherichia coli to produce the recombinant enzyme in great quantities for administration in humans (Hori et al., 1996).
Insights in nodule-inhabiting plant growth promoting bacteria and their ability to stimulate Vicia faba growth
Published in Egyptian Journal of Basic and Applied Sciences, 2022
Amr M. Mowafy, Mona S. Agha, Samia A. Haroun, Mohamed A. Abbas, Mohamed Elbalkini
The isolates P5 and P8 with close homology to Pseudomonas putida and Pseudomonas hibiscicola respectively showed the ability to produce ammonia. Additionally, the isolate P5 was able to produce IAA in addition to its ability to hydrolyze lipid, cellulose, and starch, merits that make it a potential plant growth promoting bacteria. On the other hand, the isolate P8 able to produce siderophore and showed the ability to hydrolyze all the tested macromolecules. Interestingly, both Pseudomonas putida and Pseudomonas hibiscicola were isolated from rice and regarded as potential growth-promoting endophytic bacteria for the ability to produce IAA and solubilize phosphate [34]. Several Pseudomonas species have been isolated from check pea nodules [35] although no report about the isolation of our isolates homologues from nodules. Several reports have shown the significant effect of Pseudomonas putida on rhizobia-legume symbiosis [36,37].
Three cases of retained cuff related infection after manual pull removal of peritoneal dialysis catheter
Published in Renal Failure, 2021
Suojian Zhang, Xu Zhang, Haitao Li, Zhiqiang Wei, Juan Cao
A 49-year-old man was initiated on PD treatment 6 years ago, and the primary causative disease was chronic glomerulonephritis. Owing to peritoneal ultrafiltration failure, PD treatment was stopped 6 months ago. PD catheter was removed by the ‘pull technique’. After 1 month, fluid exudation was noted at the exit site of the original PD catheter, accompanied by pain and no fever. Blood test results revealed the following: hemoglobin 118 g/L, white blood cell count 6.42 × 109/L, neutrophil percentage 78.5%, platelet count 166 × 109/L, C-reactive protein 2.56 mg/L, and procalcitonin 0.67 ng/mL. Bacterial culture of the secretion revealed the presence of Pseudomonas putida. B-mode ultrasound imaging revealed a heterogeneous echo mass in the left lower abdominal wall, measuring approximately 38 × 7 mm (Figure 2). We first administered piperacillin–sulbactam 2.5 g IV BID for 8 days without mass removal; however, the patient did not show any improvement, and B-mode ultrasound imaging indicated that the size of the mass in the left lower abdominal wall increased to approximately 45 × 8 mm (Figure 3). Abdominal wall mass resection was performed, and piperacillin–sulbactam was administered for 1 more week. Subsequently, the patient was cured.
Continuous flow system for biofilm formation using controlled concentrations of Pseudomonas putida from chicken carcass and coupled to electrochemical impedance detection
Published in Biofouling, 2020
Daoyuan Yang, José I. Reyes-De-Corcuera
Pseudomonas putida, a widely used model organism for biofilm formation (Klausen et al. 2006; Giaouris et al. 2013), was selected as a single culture to show proof of concept of the ability of the system to detect biofilm formation. Specifically, P. putida #8 from the University of Georgia’s Food Science and Technology culture collection, isolated from poultry carcass was used for this research. One frozen bead of P. putida was thawed and inoculated into 9 ml of tryptic soy broth (TSB) to grow overnight at 28 °C. Tryptic soy agar (TSA) plates were streaked with the P. putida suspension and incubated at 28 °C overnight to make cultures. Cultures were stored at 4 °C and renewed every three months. A loop of bacteria from the prepared culture was transferred into 200 ml 1/10 TSB and incubated at 28 °C with agitation (160 rpm, G24 Environment Incubator Shaker, New Brunswick Scientific, Enfield, CT) for 24 h.