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Improved treatment efficacy of risedronate functionalized chitosan nanoparticles in osteoporosis: formulation development, in vivo, and molecular modelling studies
Published in Journal of Microencapsulation, 2019
Shivalingappa Santhosh, Dhrubojyoti Mukherjee, Jayaraman Anbu, Manikanta Murahari, Banala Venkatesh Teja
The femur specimen from each group were transferred to 10% (by weight) neutral-buffered formalin solution followed by bone decalcification, dehydration, and paraffin embedding. Bone fragments of 5 mm (thickness) were separated from the diaphysis, stained with haematoxylin–eosin for microscopic analysis (Bitto et al. 2008).