China
Africa
Explore chapters and articles related to this topic
Cryostat Frozen Sections *
Published in Joseph Kovi, Hung Dinh Duong, Frozen Section In Surgical Pathology: An Atlas, 2019
Joseph Kovi, M.D. Hung Dinh Duong
Microtome knives should be sharpened whenever signs of dulling occur. Commercially available plate type microtome knife sharpeners are suitable. Good hand honing and stropping technique, however, can produce the sharpest edge.
The Nerve Cell Laid Bare
Published in Andrew P. Wickens, A History of the Brain, 2014
Purkinje’s new microscope soon led to some notable discoveries including an account of sweat glands in 1833, the first observation of ciliary motion in embryonic cells in 1834, and the recognition of the protein-digesting power of pancreatic extracts in 1836. As a reward for his dedicated work, Purkinje was presented with a second high class microscope (built by Pistor and Schiek of Berlin) in 1836, but perhaps more importantly, he received his own university building for research and lecturing in 1839. It was, in effect, the world’s first institute dedicated to microscopical research. Now known as the cradle of histology for its many discoveries, it also housed the world’s first practical microtome – a bladed instrument allowing wafer thin sections of fixed size to be cut for microscopic analysis. This instrument, first constructed under Purkinje’s leadership in 1841,was a major advance, for prior to its invention, microscopists had been forced to manually prepare tissue by using razors. The microtome now allowed reproducible clean cuts to be made of such thinness that light could shine through the tissue allowing its structure to be better visualised. This would prove to be particularly valuable in the examination of nervous material.
Tissue Staining Techniques for Stroke Studies
Published in Yanlin Wang-Fischer, Manual of Stroke Models in Rats, 2008
Yanlin Wang-Fischer, Lee Koetzner
A microtome is a device used for cutting sections. This device is usually combined with a temperature controller and a water tank. A microtome that slides the specimen across the block is called a sliding microtome. The microtome may be different in each laboratory; rotary microtomes use a rotary actuator to advance the specimen across the blade. The basic theories are the same. We introduce the system from our laboratory as a sample; it includes a water pump and tank unit (PTU-3), temperature controller (Physitemp BFS-30 TC), and microtome (HM 450) (Figure 20.3). This system was purchased from Physitemp Instruments, and with their permission we present some information about it from their Web site (Figure 20.3) (154 Huron Avenue, Clifton, New Jersey 07013, 973–779–5577, 800–452–8510, [email protected]).
Effect of PEGylation on drug uptake, biodistribution, and tissue toxicity of efavirenz–ritonavir loaded PAMAM G4 dendrimers
Published in Pharmaceutical Development and Technology, 2023
Rohini Kharwade, Nilesh Mahajan, Sachin More, Amol Warokar, Sachin Mendhi, Akshay Dhobley, Devendra Palve
RP-HPLC: LC-20 AD was obtained from Shimadzu (Kyoto, Japan). UV spectrophotometer: UV-1800 was obtained from Shimadzu (Kyoto, Japan). Rotary evaporator was obtained from Buchi Rotavapor R-100 (Buchi, Flawil, Switzerland). Cooling Centrifuge was obtained from REMI Model CM 12 Plus (REMI, Mumbai, India). Lyophilizer was obtained from MAC Lyophilizer (Freeze Dryer) (cat. no.: MSW-137) serial no. 2511 was obtained from Macro Scientific Works Pvt. Ltd. (Delhi, India). Mass spectrophotometer: Synapt XS HDMS, UPLC Acquity H class series system was obtained from Waters (Wilmslow, UK). FE-SEM: JSM-6380A Scanning Electron Microscope; 200 kV was obtained from JEOL Ltd. (Tokyo, Japan). TEM: JEM-2100 Plus Electron Microscope; 200 kV was obtained from JEOL Ltd. (Tokyo, Japan). DSC: Mettler Toledo, DSC1 was obtained from Star e-software (Ramsen, Switzerland). XRD: powder X-ray diffractometer with Ni-filtered, Bruker D2 phaser, 2nd generation was obtained from Bruker (Karlsruhe, Germany). CO2 incubator was obtained from Thermo Scientific Inc. (Waltham, MA). ELISA microplate reader: Benesphera E2 (110–250 V; OSRAM 64607) was obtained from Benesphera (Center Valley, PA). Rotary microtome: Leica Biosystem, Semiautomatic Histocore Rotary Microtome; was obtained from Leica Biosystem (Wetzlar, Germany). Optical microscope: Cilika BT-E (2021); Benchtop Biological Digital microscope was obtained from Medprime Technology Private Ltd. (India).
Morphological Changes in the Lacrimal Gland of Anophthalmic Socket in Relation to the Contralateral Normal Eye in Male Wistar Albino Rats: A Histopathology Study
Published in Current Eye Research, 2021
Dantapuram Shashidhar, Rajesh Thangarajan, Nagarajan Theruveethi, Subhashini Bonkuri
Post fixation, tissues were dehydrated with graded alcohol, then wax blocks were prepared by embedding the tissues in high-quality paraffin and preserved in 4°C till further use. Sections with a thickness of 6 µm were cut using a rotary microtome (Leica), in the form of ribbons and mounted on gelatin-coated microscopic slides.28 These sections were stained with hematoxylin and eosin as follows: after deparaffinization with xylene, sections were hydrated with descending grades of alcohol and stained with hematoxylin and eosin. Following dehydration with ascending grades of alcohol and clearing with xylene, sections were cover-slipped with DPX (Distyrene Plasticizer Xylene).29 Slides were then coded and blinded. Histopathological evaluation of the stained sections was done by a pathologist.
Effect of the Ileum and Colon on Liver Regeneration
Published in Journal of Investigative Surgery, 2021
Cláudia Nunes Oliveira, Ítalo Medeiros Azevedo, Keyla Borges Ferreira Rocha, Eryvaldo Sócrates Tabosa Egito, Aldo Cunha Medeiros
The liver samples were fixed in 10% buffered formalin for 48 hours. After fixation, they were sectioned to a thickness of 5 mm and cut with punch-type equipment (6 mm in diameter) in order to standardize the samples, then treated for 18 hours in an automatic tissue processor (Leica TP 1020, Germany). The histological sections were obtained with a microtome (Leica RM 2125 RTS, Germany), at a thickness of 3 microns, on previously silanized slides. Samples were submitted to immunohistochemical reaction with Ki67 using Anti-Ki67 antibody [SP6 ab16667] kits (ABCAM, Massachusetts, USA). The sections were counterstained with hematoxylin. The hepatocyte labeling index with Ki-67 was determined by evaluating 100 hepatocytes from the 6 highest positive fields at high power (400x), and the percentage of positive nuclei was expressed as the Ki-67 labeling index. Immunohistochemical analysis was performed in a CX41 optical microscope (Olympus, Tokyo, Japan).