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Ultrafast Fiber Lasers
Published in Iniewski Krzysztof, Integrated Microsystems, 2017
Recently, efforts to develop compact multiphoton systems for in vivo imaging and clinical applications use fs pulses from ultrafast lasers coupled into double-clad PCFs or hollow-core PBFs. Fiber-connected miniature scanning probes have been developed to provide flexible access to tissue and to acquire multiphoton microscopy (MPM) images. While the probes are becoming smaller and smaller, the sources they use are often traditional solid-state Ti:sapphire lasers that are bulky, expensive, not portable, and require precise alignment. Finding new ultrafast laser sources that are low-cost, compact, and portable is expected to enhance the impact of MPM in research and particularly in clinical studies.
Data Collection Methods
Published in Kitsakorn Locharoenrat, Research Methodologies for Beginners, 2017
A fluorescence target of 6 mm in diameter was performed with different target positions (4.5, 7.5, or 15 mm, apart from phantom surface) and concentrations (0.5, 1.0, and 2.0 mM indocyanine green dye in 1% Intralipid solution). This target was filled in a tissue phantom in which the top view is shown in Fig. 1. The target was then excited by incident light at position A (90°). The light source was a Ti:Sapphire laser with a central wavelength of 780 nm. Fluorescence light was detected by a photomultiplier. Detection points were focused at the symmetrical point B (30°) and D (−30°), as shown in Fig. 1.
Application of Nonlinear Microscopy in Life Sciences
Published in Lingyan Shi, Robert R. Alfano, Deep Imaging in Tissue and Biomedical Materials, 2017
Zdenek Svindrych, Ammasi Periasamy
Spectral shaping of ultrafast pulses is another advanced concept, where the phase of a broadband pulse is engineered in such a way, that it selectively excites one fluorophore, and not the other [47, 48]. While simple tuning of Ti:sapphire laser to appropriate wavelengths may perform similarly, spectral shapers are much faster, at can “switch” between fluorophores in milliseconds (when based on liquid crystals) or even microsecond (when based on acousto-optic interaction); typical Ti:sapphire laser can sweep across its tuning range in 10 s.
Engineering polymeric nanocapsules for an efficient drainage and biodistribution in the lymphatic system
Published in Journal of Drug Targeting, 2019
Ana Sara Cordeiro, José Crecente-Campo, Belén L. Bouzo, Santiago F. González, María de la Fuente, María José Alonso
For the 3D reconstruction imaging studies experiments, popliteal and lumbar lymph nodes of the mice being studied were harvested at 12 h post-injection and kept in PBS at 4 °C. One axillary lymph node was also collected at the same time as a control. Lymph node imaging was done using a customised 2-photon platform (TrimScope, LaVision BioTec GmbH; Bielefeld, Germany). 2-Photon excitation of the fluorescent probes was achieved using two tuneable Ti:Sapphire lasers with an output wavelength in the range of 690–1080 nm (Chamaleon Ultra I, Chamaleon Ultra II, Coherent Inc.; Santa Clara, CA), and an optical parametric oscillator emitting in the range of 1010–1340 nm (Chamaleon Compact OPO, Coherent Inc.; Santa Clara, CA). The objective used to obtain 3 D whole lymph node reconstructions was a Nikon Plan Apo λ 10×/0.45, with a mosaic of up to 4 × 3 adjacent field-of-view image acquisitions.
Enhancing osteogenic potential of hDPSCs by resveratrol through reducing oxidative stress via the Sirt1/Nrf2 pathway
Published in Pharmaceutical Biology, 2022
Jingying Zhang, Rui Li, Kenny Man, Xuebin B. Yang
The excitation source of the SHG microscope was used to be pumped by a mode-locked Ti:sapphire laser oscillator (Spectra-Physics, 80 fs, 80 MHz and average powers up to 2.9 W). The laser beam was coupled into a multiphoton fluorescence scanning microscope (BX61 + FV1200, Olympus, Tokyo, Japan). The femtoseconds laser beam was focussed onto the sample by the objective lens (10× U PlanSApo, 0.40 N.A.; Olympus, Tokyo, Japan) with 10 mW. The SHG signal was isolated from the fundamental and any fluorescence by a band-pass filter (400/10 nm) and detected using a photomultiplier tube in the backscattered light path. Images were 800 × 800 pixels with 2 μs/pixel dwell time.