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A Positive Correlation Between IgG2 Antibody Preponderance and Clearance of HSV During Active HSV Infections
Published in P. Mereena Luke, K. R. Dhanya, Didier Rouxel, Nandakumar Kalarikkal, Sabu Thomas, Advanced Studies in Experimental and Clinical Medicine, 2021
K. Vasanthi, G. Sathya Narayanan, Pugalendhi, Elanchezhiyan Manickan
A clot from the whole blood has been removed by centrifuging at 1500 g for 10 min in a refrigerated centrifuge. The resulting supernatant was designated serum. Following centrifugation, the liquid component (serum) has been immediately transferred into a clean polypropylene (PP) tube using a Pasteur pipette. The samples were maintained at 2–8°C while handling and immediately analyzed, avoiding freeze-thaw cycles because this is detrimental to many serum components.
Nucleated Cell Separation Using the Fenwal CS3000™
Published in Adrian P. Gee, BONE MARROW PROCESSING and PURGING, 2020
Herbert M. Cullis, Ellen Areman, Charles S. Carter
This procedure does not remove platelets. Platelets can be removed by: Centrifuge with the CS3000 at 1000 rpm for 3 to 4 min, orCentrifuging in a Sorval RC-3 or equivalent at 2000 rpm for 7 min.
The Hematologic System and its Disorders
Published in Walter F. Stanaszek, Mary J. Stanaszek, Robert J. Holt, Steven Strauss, Understanding Medical Terms, 2020
Walter F. Stanaszek, Mary J. Stanaszek, Robert J. Holt, Steven Strauss
The first tests usually performed are the complete blood cell count (CBC) and hematocrit (Hct). Hematocrit is determined by centrifuging blood in a graduated tube; the graduations allow measurement of the volume of the packed red cells as compared to the total volume. The CBC is actually a very rudimentary test, offering only a count of the total number of cells in a cubic milliliter of the blood.
Increased brain uptake of pterostilbene loaded folate modified micellar delivery system
Published in Drug Delivery, 2022
Yinan Wang, Yanan Su, Yunqiao Yang, Huan Jin, Moli Wu, Qian Wang, Pengyuan Sun, Jianbin Zhang, Xiaobo Yang, Xiaohong Shu
Folate modified pterostilbene loaded PEG2000/PEG3400-PCL2000 micelles (F-Pt/M) were formulated using a thin-film hydration method (Zheng et al., 2015; Mei et al., 2019). In brief (see Figure 1), Pt, PEG2000-PCL2000 and Folate-PEG3400-PCL2000 of various concentrations and proportions were dissolved in acetone in a round-bottom flask. The organic solvent was removed at 45 °C by vacuum rotary evaporation to form a dry film at the bottom of the flask. The obtained film was further dried overnight under vacuum to remove any traces of remaining acetone. After adding the preheated PBS (55 °C) to hydrate the film, the system was vortexed and sonicated for 10 min, respectively. Free unentrapped Pt was removed via using centrifugation method. Separation was carried out by centrifuging at 10,000 rpm for 10 min (Centrifuge H1650-W, Xiangyi, Hunan). Finally, the supernatant was extruded through a polycarbonate membrane of 220 nm pore size for 10 times using a laboratory extrusion device (LiposoFast Basic LF-1, Avestin Inc., Ottawa, Canada). In addition, blank micelles (BM) and pterostilbene loaded micelles (Pt/M) were prepared via the same way. To study the uptake of folate modified micelles by cancer cells, the coumarin-6 (C6), a fluorescent material, loaded micelles (C6/M and F-C6/M) were prepared as well.
Point-of-care viral load testing among adolescents and young adults living with HIV in Haiti: a randomized control trial
Published in AIDS Care, 2022
Lindsey K. Reif, Marie Elmase Belizaire, Vanessa Rouzier, Grace Seo, Patrice Severe, Joseph-Marie Bajo Joseph, Bernadette Joseph, Sandra Apollon, Jean W. Pape, Margaret L. McNairy, Batya Elul, Daniel W. Fitzgerald, Stephen M. Arpadi, Elaine J. Abrams, Louise Kuhn
All VL tests for participants in the POC arm were performed on the Cepheid GeneXpert system using Xpert HIV-1 Viral LoadTM cartridges (Cepheid, Sunnyvale, CA). A GeneXpert 4-module instrument was placed at the on-site laboratory and operated by laboratory technicians. This system uses individual cartridges which perform integrated extraction and quantitative real-time PCR for measurement of HIV-1 RNA. Processing the assay includes centrifuging the blood sample for 15 minutes to separate plasma, and then 90 minutes of run-time. Participants were asked to arrive before 11 am to allow time for same-day processing. When VL test results were ready, the laboratory technician called the study nurse for pick-up and the study nurse provided the result coupled with adherence counseling to the participant. If the result was not available the same-day, the participant returned for the result and adherence counseling as soon as available.
Comparison of Centrifugal and Pulsatile Perfusion to Preserve Donor Kidneys Using Ex Vivo Subnormothermic Perfusion
Published in Journal of Investigative Surgery, 2022
Patrick P. W. Luke, Larry Jiang, Aushanth Ruthirakanthan, Daniel Lee, Qizhi Sun, Mahms Richard-Mohamed, Justin Kwong, Shahid Aquil, Rafid Alogaili, Aaron Haig, Alp Sener, Rabindra N. Bhattacharjee
We determined the amount of neutrophil gelatinase-associated lipocalin 1 (NGAL 1) (MyBioSource, CA, USA) and IL-6 (Abcam, Cambridge, UK) in urine according to the supplied protocol. Briefly, urine samples were pre-cleared by centrifuging 5 min at 3,000rpm. 100 μL of standards and urine samples were used to incubate in the pre-coated ELISA plate for 2 hr. After wash, 100 μL of biotinylated detection antibodies were added to the plate and incubation was continued for 1 hr. 100 μL of avidin-HRP working solution was added to the plate after wash. Following 1 hr incubation and wash, the substrate reagent was added to the plate and color was developed. The plates were read by an iMark microplate reader (BioRad, USA) at 450 nm. Concentrations of NGAL 1 and IL-6 were determined from the standard curve.