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General Biological Aspects of Oncogenesis
Published in Pimentel Enrique, Oncogenes, 2020
Spontaneous regression has also been observed in certain experimental tumors, like the malignant lymphomas induced in Swiss and NZW mice by 1-ethyl-1-nitrosourea (ENU) and the leukemia induced in chickens with avian myeloblastosis virus (AMV).285,286 The mechanisms involved in spontaneous regression of tumors are multiple and may be different in different cases but one mechanism may consist in the induction of terminal differentiation by unidentified endogenous and/or exogenous environmental stimuli. It is likely that only the tumors originated in partially differentiated cells can undergo spontaneous regression due to terminal differentiation.
Embryonic and Fetal Erythropoiesis
Published in Stephen A. Feig, Melvin H. Freedman, Clinical Disorders and Experimental Models of Erythropoietic Failure, 2019
D. Wade Clapp, Kevin M. Shannon
The c-myb proto-oncogene is the cellular homologue of the v-myb gene of avian myeloblastosis virus (AMV). The v-myb oncogene transforms myeloid cells in culture and causes myelomonocytic leukemia in chickens; v-myb is truncated at its 5′ and 3′ ends relative to c-myb. The c-myb protein includes a conserved DNA binding domain at its 5′ end and a downstream “leucine zipper” motif, which is thought to mediate protein-protein interactions.74
A Survey of Newer Gene Probing Techniques
Published in Victor A. Bernstam, Pocket Guide to GENE LEVEL DIAGNOSTICS in Clinical Practice, 2019
While the TAS system requires temperature shifts for optimal reaction rates at the DNA synthesis and RNA transcription steps, a modification of this system using three different enzymes is an isothermal process. The 3SR system in essence reproduces the strategy employed by retroviral replication. The three enzymes carrying out the cDNA synthesis and RNA transcription simultaneously are as follow: avian myeloblastosis virus (AMV) reverse transcriptaseEscherichia coliRNase H, dissociating RNA-DNA duplexesT7 RNA polymerase
Cardioprotective Efficacy of Coriandrum sativum (L.) Seed Extract in Heart Failure Rats Through Modulation of Endothelin Receptors and Antioxidant Potential
Published in Journal of Dietary Supplements, 2020
Neha Dhyani, Adila Parveen, Aisha Siddiqi, M. Ejaz Hussain, Mohammad Fahim
The expression of ETA and ETB receptors mRNA was analyzed by RT-PCR. The expression of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA was determined as an internal control. Total tissue RNA was isolated by acid guanidinium thiocyanate-phenol-chloroform extraction with Isogen according to the manufacturer’s instructions. The tissue was homogenized in Trizol (0.2 g tissue per 2 ml Trizol) with a tissue homogenizer. Chloroform extraction, isopropanol precipitation, and 75% (v/v) ethanol washing of precipitated RNA were subsequently performed. The obtained RNA was resolved in diethyl pyrocarbonate–treated water. The RNA concentration was measured using nanodrop. Total RNA (5 µg) was primed with 0.05 µg oligo-d(T) 12–18 and reverse transcribed by avian myeloblastosis virus reverse transcriptase using the First-Strand cDNA Synthesis Kit. The obtained cDNA was diluted in a 1:10 ratio, and 1 µl was used for real-time PCR.
Silencing long non-coding RNA HNF1A-AS1 inhibits growth and resistance to TAM of breast cancer cells via the microRNA-363/SERTAD3 axis
Published in Journal of Drug Targeting, 2021
Ying Li, Ling Liu, Yidong Lv, Yanwu Zhang, Linlin Zhang, Haiyang Yu, Weifang Tian, Zhan Zhang, Shihong Cui
Total RNA of cells was extracted using Trizol (Invitrogen). Ultraviolet analysis and formaldehyde denaturing gel electrophoresis were applied to determine the high quality of obtained RNA. Next, l μg RNA was reverse transcribed into cDNA using avian myeloblastosis virus reverse transcriptase. The SYBR Green method was used for qPCR. PCR primers (Table 1) were synthesised by Sangon (Shanghai, China). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and U6 were set as the internal reference. Data were analysed using 2−ΔΔCt method in which ΔΔCt = [Ct (target gene) − Ct (internal control)]experimental group − [Ct (target gene) − Ct (internal control)]control group.
Vitamin C Prevents Hydrocortisone-Induced Injury in HMEC-1 through Promoting Bestrophin-3 Expression
Published in Nutrition and Cancer, 2019
Xuexin Wang, Guoping Zhang, Chaohua Zhu, Lei Lin, Zhenshuan Zhao, Xiaoguang Yu, Guobin Liu, Haijing Zhang, Quanhai Li, Wei Dong, Jian Wang
Total RNA was extracted from the cells using Trizol reagent (Life Technologies, Carlsbad, CA). An aliquot of 1 μg total RNA was reversely transcribed by an avian myeloblastosis virus reverse-transcriptase kit (Promega, Madison, WI). The expression of target genes was normalized to GAPDH. The primers were listed as follows: